Construct: ORF TRCN0000473854
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007901.1_s317c1
- Derived from:
- ccsbBroadEn_05870
- DNA Barcode:
- ACACGTATAGCGAAAGAGTCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5MC1 (516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473854
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 516 | ATP5MC1 | ATP synthase membrane subun... | NM_001002027.2 | 99.5% | 99.2% | 11C>G;303G>T |
| 2 | human | 516 | ATP5MC1 | ATP synthase membrane subun... | NM_005175.3 | 99.5% | 99.2% | 11C>G;303G>T |
| 3 | mouse | 11951 | Atp5g1 | ATP synthase, H+ transporti... | NM_001161419.1 | 89.2% | 89.7% | (many diffs) |
| 4 | mouse | 11951 | Atp5g1 | ATP synthase, H+ transporti... | NM_007506.6 | 89.2% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 498
- ORF length:
- 408
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCAGACCGG CGGGGCATTA TTCATTTCTC 121 CAGCTCTGAT CCGCTGTTGT ACCAGGGGTC TAATCAGGCC TGTGTCTGCC TCCTTCTTGA 181 ATAGCCCAGT GAATTCATCT AAACAGCCTT CCTACAGCAA CTTCCCACTC CAGGTGGCCA 241 GACGGGAGTT CCAGACCAGT GTTGTCTCCC GGGACATTGA CACAGCAGCC AAGTTTATTG 301 GTGCTGGGGC AGCCACAGTT GGTGTGGCTG GTTCAGGGGC TGGCATTGGA ACCGTGTTTG 361 GCAGCTTGAT CATTGGCTAT GCCAGGAACC CTTCTCTCAA GCAGCAGCTC TTCTCCTATG 421 CCATTCTTGG CTTTGCCCTG TCTGAGGCCA TGGGGCTTTT CTGTTTGATG GTCGCCTTCC 481 TCATCCTCTT CGCCATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 541 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 601 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACACGTA TAGCGAAAGA 661 GTCCCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt