Construct: ORF TRCN0000473856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017720.1_s317c1
- Derived from:
- ccsbBroadEn_02321
- DNA Barcode:
- GACATCCTTCTTCCGGAGAAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL4C (10123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10123 | ARL4C | ADP ribosylation factor lik... | NM_005737.3 | 100% | 100% | |
| 2 | human | 10123 | ARL4C | ADP ribosylation factor lik... | NM_001282431.2 | 95.5% | 95.5% | 577_603del |
| 3 | mouse | 320982 | Arl4c | ADP-ribosylation factor-lik... | NM_177305.4 | 95.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 645
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaacatc tcctctaaca tctcggcctt ccagtccctg catatcgtca 121 tgttgggctt ggactcggcc ggcaagacca cggtgctcta ccggctcaag ttcaacgagt 181 tcgtgaacac ggtgcccacc atcggcttca acaccgagaa gatcaagctg agcaacggca 241 cggccaaggg caTCAGCTGC CACTTCTGGG ACGTGGGCGG CCAGGAGAAG CTGCGGCCGC 301 TGTGGAAGTC CTACAGCCGC TGCACGGACG GCATCATCTA CGTGGTGGAC TCGGTGGACG 361 TGGACCGGCT GGAGGAGGCC AAGACGGAGC TGCACAAGGT GACCAAGTTC GCCGAGAACC 421 AGGGCACGCC GCTGCTGGTC ATCGCCAACA AGCAGGACCT GCCCAAGTCG CTGCCGGTGG 481 CAGAGATTGA GAAGCAGCTG GCGCTGCACG AGCTTATCCC GGCCACCACC TATCACGTCC 541 AGCCGGCGTG CGCCATCATC GGCGAGGGCC TCACCGAGGG CATGGACAAG CTCTATGAGA 601 TGATCCTGAA ACGCAGGAAG TCCCTCAAGC AGAAGAAGAA GCGGTTGCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA GACATCCTTC TTCCGGAGAA ATGTACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt