Construct: ORF TRCN0000473864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017828.1_s317c1
- Derived from:
- ccsbBroadEn_01610
- DNA Barcode:
- AGATCCTCAGTTGCCCGGATGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAT4 (6775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473864
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6775 | STAT4 | signal transducer and activ... | NM_001243835.2 | 100% | 100% | |
| 2 | human | 6775 | STAT4 | signal transducer and activ... | NM_003151.4 | 100% | 100% | |
| 3 | human | 6775 | STAT4 | signal transducer and activ... | XM_006712719.3 | 100% | 100% | |
| 4 | human | 6775 | STAT4 | signal transducer and activ... | XM_011511705.2 | 100% | 100% | |
| 5 | human | 6775 | STAT4 | signal transducer and activ... | XM_017004784.2 | 66% | 64% | (many diffs) |
| 6 | mouse | 20849 | Stat4 | signal transducer and activ... | NM_001308266.1 | 88.3% | 94.7% | (many diffs) |
| 7 | mouse | 20849 | Stat4 | signal transducer and activ... | NM_011487.5 | 88.3% | 94.7% | (many diffs) |
| 8 | mouse | 20849 | Stat4 | signal transducer and activ... | XM_006495815.2 | 84.4% | 85.6% | (many diffs) |
| 9 | mouse | 20849 | Stat4 | signal transducer and activ... | XM_006495816.1 | 82.2% | 88.2% | (many diffs) |
| 10 | mouse | 20849 | Stat4 | signal transducer and activ... | XM_006495814.2 | 81.5% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2310
- ORF length:
- 2244
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcagtggaat caagtccaac agttagaaat caagtttttg gagcaggtgg 121 atcaattcta tgatgacaac tttcccatgg aaattcggca tctgttggcc caatggattg 181 aaaatcaaga ctgggaggca gcttctaaca atgaaaccat ggcaacgatt cttcttcaaa 241 acttgttaat acaactggat gaacagttag gtcgtgtttc caaagagaaa aacctactct 301 tgatacacaa tctaaaaaga attaggaagg tccttcaggg aaaatttcat ggaaatccaa 361 tgcatgtagc tgtggttatt tcaaactgtt taagggaaga gaggagaata ttggctgcag 421 ccaacatgcc tgtccagggg cctctagaga aatccttaca aagttcttca gtttcagaaa 481 gacagaggaa tgtggagcac aaagtggctg ccattaaaaa cagtgtgcag atgacagaac 541 aagataccaa atacttagaa gatctgcaag acgaatttga ctacaggtat aaaacaattc 601 agacaatgga tcagagtgac aagaatagtg ccatggtgaa tcaggaagtt ttgacactgc 661 aggaaatgct taacagcctc gatttcaaga gaaaggaggc tctcagtaaa atgacccaaa 721 tcatccatga gacagacctg ttaatgaaca ccatgctcat agaagagctg caagactgga 781 agcggcggca gcaaatcgcc tgcatcgggg gtccactcca caatgggctc gaccagcttc 841 agaactgctt tacactattg gcagaaagtc ttttccaact gagaaggcaa ttggagaaac 901 tagaggagca atctaccaaa atgacatatg aaggtgatcc cattccaatg caaagaactc 961 acatgctaga aagagtcacc ttcttgatct acaacctttt caagaactca tttgtggttg 1021 agcgacagcc atgtatgcca acccaccctc agaggccgtt ggtacttaaa accctaattc 1081 agttcactgt aaaactaagg ctactaataa aattgccaga actaaactat caggtaaagg 1141 ttaaggcatc aattgacaag aatgtttcaa ctctaagcaa ccgaagattt gtactttgtg 1201 gaactaatgt caaagccatg tctattgaag aatcttccaa tgggagtctc tcagtagaat 1261 ttcgacattt gcaaccaaag gaaatgaagt ccagtgctgg aggtaaagga aatgagggct 1321 gtcacatggt gactgaagaa cttcattcca taacgtttga aacacagatc tgcctctatg 1381 gcctgaccat agatttggag accagctcat tgcctgtggt gatgatttcc aatgtcagtc 1441 agttacctaa tgcttgggca tccatcattt ggtacaacgt gtcaaccaac gattcccaga 1501 acttggtttt ctttaataat cctccacctg ccacattgag tcaactactg gaggtgatga 1561 gctggcagtt ttcatcgtac gttggtcgtg gtcttaactc agatcaactc catatgctgg 1621 cagagaagct tacagtccaa tctagctaca gtgatggtca cctcacctgg gccaagttct 1681 gcaaggaaca tttacctggt aaatcattta ccttttggac atggcttgaa gcaatattgg 1741 atctaattaa gaaacacatt cttccccttt ggattgatgg gtatgtcatg ggctttgtta 1801 gcaaagagaa ggaacggctg ttgctaaagg ataaaatgcc tggcaccttt ttattaagat 1861 tcagtgaaag ccatctcgga ggaaTAACTT TCACCTGGGT GGACCATTCT GAAAGTGGGG 1921 AAGTGAGATT CCACTCTGTA GAACCCTACA ATAAAGGCCG GTTGTCTGCT CTGCCATTCG 1981 CTGACATCCT GCGAGACTAC AAAGTTATTA TGGCTGAAAA CATTCCTGAA AACCCTCTGA 2041 AGTACCTATA TCCTGACATT CCCAAAGACA AAGCCTTCGG TAAACACTAC AGCTCTCAGC 2101 CTTGCGAAGT TTCAAGACCA ACAGAAAGGG GTGACAAAGG TTATGTTCCT TCTGTTTTTA 2161 TCCCCATCTC AACAATCCGA AGTGATTCAA CAGAGCCACA TTCTCCATCA GACCTTCTTC 2221 CCATGTCTCC AAGTGTGTAT GCGGTGTTGA GAGAAAACCT GAGTCCCACA ACAATTGAAA 2281 CTGCAATGAA GTCTCCTTAT TCTGCTGAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2341 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2401 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGATC 2461 CTCAGTTGCC CGGATGGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2521 tgaaagatt