Construct: ORF TRCN0000473876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005248.1_s317c1
- Derived from:
- ccsbBroadEn_07724
- DNA Barcode:
- AACGCCCTAACAGTGCAAAACAAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDELR2 (11014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11014 | KDELR2 | KDEL endoplasmic reticulum ... | NM_006854.4 | 99.8% | 100% | 405A>G |
| 2 | human | 11014 | KDELR2 | KDEL endoplasmic reticulum ... | NM_001100603.2 | 70.3% | 49.8% | (many diffs) |
| 3 | mouse | 66913 | Kdelr2 | KDEL (Lys-Asp-Glu-Leu) endo... | NM_025841.4 | 90.5% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cattttccgg ctgactgggg acctgtccca cctggcggcc atcgtcatcc 121 tgctgctgaa gatctggaag acgcgctcct gcgccggtat ttctgggaaa agccagcttc 181 tgtttgcact ggtcttcaca actcgttacc tggatctttt tacttcattt atttcattgt 241 ataacacatc tatgaaggtt atctaccttg cctgctccta tgccacagtg tacctgatct 301 acctgaaatt taaggcaacc tacgatggaa atcatgataC CTTCCGAGTG GAGTTTCTGG 361 TGGTCCCTGT GGGAGGCCTC TCATTTTTAG TTAATCACGA TTTCTCTCCT CTTGAGATCC 421 TCTGGACCTT CTCCATCTAC CTGGAGTCCG TGGCTATCCT TCCGCAGCTG TTTATGATCA 481 GCAAGACTGG GGAGGCCGAG ACCATCACCA CCCACTACCT GTTCTTCCTG GGCCTCTATC 541 GTGCTTTGTA TCTTGTCAAC TGGATCTGGC GCTTCTACTT TGAGGGCTTC TTTGACCTCA 601 TTGCTGTGGT GGCCGGCGTA GTCCAGACCA TCCTATACTG TGACTTCTTC TACTTGTACA 661 TTACAAAAGT ACTCAAGGGA AAGAAGCTCA GTTTGCCAGC AACCCAACTT TCTTGTACAA 721 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 781 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 841 GGACGAAACG CCCTAACAGT GCAAAACAAT ACGCGTTAAG TCgacaatca acctctggat 901 tacaaaattt gtgaaagatt