Construct: ORF TRCN0000473881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016450.1_s317c1
- Derived from:
- ccsbBroadEn_12217
- DNA Barcode:
- ATGAAATTCTATAAGATGGAGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCM10 (55388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55388 | MCM10 | minichromosome maintenance ... | NM_018518.5 | 40.3% | 40.2% | 1_1563del;1618A>T |
| 2 | human | 55388 | MCM10 | minichromosome maintenance ... | NM_182751.3 | 40.3% | 40.2% | 1_1566del;1621A>T |
| 3 | human | 55388 | MCM10 | minichromosome maintenance ... | XM_011519538.2 | 40.3% | 40.2% | 1_1566del;1621A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1125
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctgccgacg tgtggagcca ggaacttaaa acaacattta gccaaagcct 121 cagcttcagg gattatgggg agcccaaaac cagccatcaa gtccatctcg gcctcagcac 181 tcttgaagca acagaagcag cggatgttgg agatgaggag aaggaaatca gaagaaatac 241 agaagcgatt tctgcagagc tcaagtgaag ttgagagccc agctgtgcca tcttcatcaa 301 gacagccccc tgctcagcct ccacggacag gatccgagtt ccccaggctg gagggagccc 361 cggccacaat gacgcccaag ctggggcgag gtgtcttgga aggagatgat gttctctttt 421 atgatgagtc accaccacca agaccaaaac tgagtgcttt agcagaagcc aaaaagttag 481 ctgctatcac caaattaagg gcaaaaggcc aggttcttac aaaaacaaac ccaaacagca 541 ttaagaagaa acaaaaggac ccTCAGGACA TCCTGGAGGT GAAGGAACGT GTAGAAAAAA 601 ACACCATGTT TTCTTCTCAA GCTGAGGATG AATTGGAGCC TGCCAGGAAA AAAAGGAGAG 661 AACAACTTGC CTATCTGGAA TCTGAGGAAT TTCAGAAAAT CCTAAAAGCA AAATCAAAAC 721 ACACAGGCAT CCTGAAAGAG GCCGAGGCTG AGATGCAGGA GCGCTACTTT GAGCCACTGG 781 TGAAAAAAGA ACAAATGGAA GAAAAGATGA GAAACATCAG AGAAGTGAAG TGCCGTGTCG 841 TGACATGCAA GACGTGCGCC TATACCCACT TCAAGCTGCT GGAGACCTGC GTCAGTGAGC 901 AGCATGAATA CCACTGGCAT GATGGTGTGA AGAGGTTTTT CAAATGTCCC TGTGGAAACA 961 GAAGCATCTC CTTGGACAGA CTCCCGAACA AGCACTGCAG TAACTGTGGC CTCTACAAAT 1021 GGGAACGGGA CGGAATGCTA AAGGAAAAGA CTGGTCCAAA GATAGGAGGA GAAACTCTGT 1081 TACCAAGAGG AGAAGAACAT GCTAAATTTC TGAACAGCCT TAAATACCCA ACTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA ATGAAATTCT ATAAGATGGA GAAAACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt