Construct: ORF TRCN0000473883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017684.1_s317c1
- Derived from:
- ccsbBroadEn_01758
- DNA Barcode:
- CCGCACATCACCCTGCTTCCTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UQCRH (7388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473883
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_006004.4 | 100% | 100% | |
| 2 | human | 440567 | UQCRHL | ubiquinol-cytochrome c redu... | NM_001089591.1 | 98.5% | 96.7% | (many diffs) |
| 3 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_001297566.2 | 90.1% | 90.1% | 53_54ins27 |
| 4 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_001297565.2 | 77.2% | 60.3% | (many diffs) |
| 5 | mouse | 66576 | Uqcrh | ubiquinol-cytochrome c redu... | NM_025641.3 | 83.1% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 489
- ORF length:
- 273
- Sequence:
-
1 GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG 61 GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT 121 CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGCTAGCA 181 TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGGG ACTGGAGGAC GAGCAAAAGA 241 TGCTTACCGA ATCCGGAGAT CCTGAGGAGG AGGAAGAGGA AGAGGAGGAA TTAGTGGATC 301 CCCTAACAAC AGTGAGAGAG CAATGCGAGC AGTTGGAGAA ATGTGTAAAG GCCCGGGAGC 361 GGCTAGAGCT CTGTGATGAG CGTGTATCCT CTCGATCACA TACAGAAGAG GATTGCACGG 421 AGGAGCTCTT TGACTTCTTG CATGCGAGGG ACCATTGCGT GGCCCACAAA CTCTTTAACA 481 ACTTGAAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGCAC ATCACCCTGC TTCCTGCGAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt