Construct: ORF TRCN0000473890
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018830.2_s317c1
- Derived from:
- ccsbBroadEn_15338
- DNA Barcode:
- TGACTAACAATGTAAGAAATTGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC652799 (652799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473890
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008180.1 | 24.8% | 24.9% | 1_951del;1676_2913del |
| 2 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_001093772.1 | 24.8% | 24.8% | 1_951del;1676_2916del |
| 3 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008179.1 | 24.8% | 24.8% | 1_954del;1679_2916del |
| 4 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265742.3 | 24.8% | 24.8% | 1_954del;1679_2919del |
| 5 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008178.1 | 24.7% | 24.8% | 1_951del;1525_1536del;1688_2925del |
| 6 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2 | 24.7% | 24.7% | 1_951del;1525_1536del;1688_2928del |
| 7 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265741.1 | 24.7% | 24.7% | 1_954del;1528_1539del;1691_2928del |
| 8 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265740.1 | 24.7% | 24.7% | 1_954del;1528_1539del;1691_2931del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 795
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gataaacact acagtatttg taaacgatgg agaaaatgta gatttgattg 121 ttgaatatga agcattcccc aaacctgaac accagcagtg gatctatatg aacagaacct 181 tcactgataa atgggaagat tatcccaagt ctgagaatga aagtaatatc agatacgtaa 241 gtgaacttca tctaacgaga ttaaaaggca ccgaaggagg cacttacaca ttcctAGTGT 301 CCAATTCTGA CGTCAATGCT GCCATAGCAT TTAATGTTTA TGTGAATACA AAACCAGAAA 361 TCCTGACTTA CGACAGGCTC GTGAATGGCA TGCTCCAATG TGTGGCAGCA GGATTCCCAG 421 AGCCCACAAT AGATTGGTAT TTTTGTCCAG GAACTGAGCA GAGATGCTCT GCTTCTGTAC 481 TGCCAGTGGA TGTGCAGACA CTAAACTCAT CTGGGCCACC GTTTGGAAAG CTAGTGGTTC 541 AGAGTTCTAT AGATTCTAGT GCATTCAAGC ACAATGGCAC GGTTGAATGT AAGGCTTACA 601 ACGATGTGGG CAAGACTTCT GCCTATTTTA ACTTTGCATT TAAAGAGCAA ATCCATCCCC 661 ACACCCTGTT CACTCCTTTG CTGATTGGTT TCGTAATCGT AGCTGGCATG ATGTGCATTA 721 TTGTGATGAT TCTGACCTAC AAATATTTAC AGAAACCCAT GTATGAAGTA CAGTGGAAGG 781 TTGTTGAGGA GATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 841 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 901 TCGATTTCTT GGCTTTATAT ATCTTGGAAA GGACGATGAC TAACAATGTA AGAAATTGGA 961 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt