Construct: ORF TRCN0000473901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010434.1_s317c1
- Derived from:
- ccsbBroadEn_03111
- DNA Barcode:
- TGTACTCGCCCTGCGTCCTGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPA4 (29935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29935 | RPA4 | replication protein A4 | NM_013347.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag taagagtggg tttgggagct atggcagcat ttctgctgct gatggagcga 121 gtggaggcag tgaccaactg tgtgagagag atgcaactcc tgctattaag acccaaagac 181 ctaaggtccg aattcaggac gttgtaccgt gtaatgtgaa ccagcttctc agctctactg 241 tgtttgaccc tgtgttcaag gttaggggaa ttatagtttc ccaggtctcc atcgtggggg 301 taatcagagg ggcagagaag gcttcaaatc acatttgtta caaaattgat gatatgaccg 361 cgaaaccaat cgaggcccga cagtggTTTG GTAGAGAGAA AGTCAAGCAG GTGACTCCAT 421 TGTCAGTCGG AGTATATGTC AAAGTGTTTG GTATCCTCAA ATGTCCCACG GGAACAAAGA 481 GCCTTGAGGT ATTGAAAATT CATGTCCTAG AGGACATGAA CGAGTTCACC GTGCATATTC 541 TGGAAACGGT CAATGCACAC ATGATGCTGG ATAAAGCCCG TCGTGATACC ACTGTAGAAA 601 GTGTGCCTGT GTCTCCATCA GAAGTGAATG ATGCTGGGGA TAACGATGAG AGTCACCGCA 661 ATTTCATCCA GGACGAAGTG CTGCGTTTGA TTCATGAGTG TCCTCATCAG GAAGGGAAGA 721 GCATCCATGA GCTCCGGGCT CAGCTCTGCG ACCTTAGCGT CAAGGCCATC AAGGAAGCGA 781 TTGATTATCT GACCGTTGAG GGCCACATCT ATCCCACTGT GGATCGGGAG CATTTTAAGT 841 CTGCTGATTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTACT CGCCCTGCGT CCTGCCTCAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt