Construct: ORF TRCN0000473904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002187.1_s317c1
- Derived from:
- ccsbBroadEn_10790
- DNA Barcode:
- TACTGAATTCCTACAGGTACCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DVL1 (1855)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1855 | DVL1 | dishevelled segment polarit... | NM_004421.3 | 66.2% | 66.1% | 366A>G;647_1324del |
| 2 | human | 1855 | DVL1 | dishevelled segment polarit... | NM_001330311.2 | 63.8% | 63.7% | 366A>G;647_1399del |
| 3 | human | 1855 | DVL1 | dishevelled segment polarit... | XM_005244733.4 | 47.7% | 47.6% | (many diffs) |
| 4 | human | 1855 | DVL1 | dishevelled segment polarit... | XM_005244732.4 | 46% | 45.8% | (many diffs) |
| 5 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538521.3 | 59.7% | 64.3% | (many diffs) |
| 6 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538520.3 | 57.4% | 61.8% | (many diffs) |
| 7 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538519.3 | 56.3% | 60.5% | (many diffs) |
| 8 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | NM_001302342.1 | 55.4% | 59.6% | (many diffs) |
| 9 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | NM_010091.4 | 55.4% | 59.6% | (many diffs) |
| 10 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538518.3 | 54.3% | 58.4% | (many diffs) |
| 11 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538522.3 | 41.5% | 42.7% | (many diffs) |
| 12 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_017319952.1 | 40.8% | 43.8% | (many diffs) |
| 13 | mouse | 13542 | Dvl1 | dishevelled segment polarit... | XM_006538523.3 | 40% | 43% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagaccaag attatctacc acatggacga ggaggagacg ccgtacctgg 121 tcaagctgcc cgtggccccc gagcgcgtca cgctggccga cttcaagaac gtgctcagca 181 accggcccgt gcacgcctac aaattcttct ttaagtccat ggaccaggac ttcggggtgg 241 tgaaggagga gatctttgat gacaatgcca agcttccctg cttcaacggc cgcgtggtct 301 cctggctggt cctggctgag ggtgctcact cggatgcggg gtcccagggc acggacagcc 361 acacagacct gcccccgcct cttgagcgga caggcggcat cggggactcc cggcccccct 421 ccttccaccc gaatgtggcc agcagccgtg acgggatgga caacgagaca ggcacggagt 481 ccatggtcag tcaccggcgg gagcgtgccc gacgccggaa ccgcgaggag gccgcccgga 541 ccaatgggca cccaagggga gaccgacggc gggatgtggg gctgccccca gacagcgcgt 601 ccaccgccct cagcagcgag cttgagtcca gcagctttgt ggactcggac gaggatggca 661 gcacgagcag gctcagcagc tccacggagc agagcacctc atccagactc acccggaagt 721 acgccagcag cttgctgaag cacggcttcc tgcggcacac ggtcaacaag atcaccttct 781 ccgagcagtg ctactacgtc ttcggggatc tctgcagcaa tctcgccacc ctgaacctca 841 acagtggctc cagtgggact tcggatcagg acacgctggc cccgctgccc cacccggctg 901 ccccctggcc tctgggtcag ggctacccct accagtaccc gggaccccca ccctgcttcc 961 cgcctgccta ccaggacccg ggctttagct atggcagcgg cagcaccggg agtcagcaga 1021 gtgaagggag caaaagcagt gggtccaccc ggagcagccg ccgggccccg ggccgtgaga 1081 aggagcgtcg ggcggcggga gctgggggca gtggcagtga atcggatcac acggcaccga 1141 gtggggtggg gagcagctgg cgagagcgtc cggccggcca gctcagccgt ggcagcagcc 1201 cacgcagtca ggcctcggct accgccccGG GGCTCCCCCC GCCCCACCCC ACGACCAAGG 1261 CCTATACAGT GGTGGGGGGG CCACCCGGGG GACCCCCTGT CCGGGAGCTG GCTGCCGTCC 1321 CCCCGGAATT GACAGGCAGC CGCCAGTCCT TCCAGAAGGC TATGGGGAAC CCCTGCGAGT 1381 TCTTCGTGGA CATCATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACTGAA TTCCTACAGG 1561 TACCGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt