Construct: ORF TRCN0000473925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002846.1_s317c1
- Derived from:
- ccsbBroadEn_05908
- DNA Barcode:
- AATCCTCACGCTGGACTAGGTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPO (706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473925
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 706 | TSPO | translocator protein | NM_000714.6 | 99.6% | 98.8% | 439A>G;485G>A |
| 2 | human | 706 | TSPO | translocator protein | NM_001256530.1 | 99.6% | 98.8% | 439A>G;485G>A |
| 3 | human | 706 | TSPO | translocator protein | NM_001256531.1 | 99.6% | 98.8% | 439A>G;485G>A |
| 4 | human | 706 | TSPO | translocator protein | NR_046308.2 | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccgccctgg gtgcccgcca tgggcttcac gctggcgccc agcctggggt 121 gcttcgtggg ctcccgcttt gtccacggcg agggtctccg ctggtacgcc ggccTGCAGA 181 AGCCCTCGTG GCACCCGCCC CACTGGGTGC TGGGCCCTGT CTGGGGCACG CTCTACTCAG 241 CCATGGGGTA CGGCTCCTAC CTGGTCTGGA AAGAGCTGGG AGGCTTCACA GAGAAGGCTG 301 TGGTTCCCCT GGGCCTCTAC ACTGGGCAGC TGGCCCTGAA CTGGGCATGG CCCCCCATCT 361 TCTTTGGTGC CCGACAAATG GGCTGGGCCT TGGTGGATCT CCTGCTGGTC AGTGGGGCGG 421 CGGCAGCCAC TACCGTGGCC TGGTACCAGG TGAGCCCGCT GGCCGCCCGC CTGCTCTACC 481 CCTACCTGGC CTGGCTGGCC TTCGCGACCA CACTCAACTA CTGCGTATGG CGGGACAACC 541 ATGGCTGGCA TGGGGGACGG CGGCTGCCAG AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 721 TCCTCACGCT GGACTAGGTG CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt