Construct: ORF TRCN0000473929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001739.1_s317c1
- Derived from:
- ccsbBroadEn_10900
- DNA Barcode:
- TTGGGTCCACCCGTTTGTTTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFI (3426)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3426 | CFI | complement factor I | XM_006714210.4 | 68% | 66.2% | (many diffs) |
| 2 | human | 3426 | CFI | complement factor I | XM_017008166.2 | 67.5% | 65.7% | (many diffs) |
| 3 | human | 3426 | CFI | complement factor I | XM_017008164.2 | 66% | 64.3% | (many diffs) |
| 4 | human | 3426 | CFI | complement factor I | XM_011531920.2 | 65.1% | 63.4% | (many diffs) |
| 5 | human | 3426 | CFI | complement factor I | XM_017008165.2 | 64.8% | 63.2% | (many diffs) |
| 6 | human | 3426 | CFI | complement factor I | NM_000204.4 | 62% | 60.5% | (many diffs) |
| 7 | human | 3426 | CFI | complement factor I | NM_001318057.2 | 61.1% | 59.5% | (many diffs) |
| 8 | human | 3426 | CFI | complement factor I | NM_001331035.2 | 60.8% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcttcttcat gttttcctgt tatttctgtg cttccactta aggttttgca 121 aggtcactta tacatctcaa gaggatctgg tggagaaaaa gtgcttagca aaaaaatata 181 ctcacctctc ctgcgataaa gtcttctgcc agccatggca gagatgcatt gagggcacct 241 gtgtttgtaa actaccgtat cagtgcccaa agaatggcac tgcagtgtgt gcaactaaca 301 ggagaagctt cccaacatac tgtcaacaaa agagtttgga atgtcttcat ccagggacaa 361 agtttttaaa taacggaaca tgcacagccg aaggaaagtt tagtgtttcc ttgaagcatg 421 gaaatacaga ttcagaggga atagttgaag taaaacttgt ggaccaagat aagacaatgt 481 tcatatgcaa aagcagctgg agcatgaggg aagccaacgt ggcctgcctt gaccttgggt 541 ttcaacaagg tgctgatact caaagaaggt ttaagttgtc tgatctctct ataaattcca 601 ctgaatgtct acatgtgcat tgccgaggat tagagaccag tttggctgaa tgtactttta 661 ctaagagaag aactatgGGT TACCAGGATT TCGCTGATGT GGTTTGTTAT ACACAGAAAG 721 CAGATTCTCC AATGGATGAC TTCTTTCAGT GTGTGAATGG GAAATACATT TCTCAGATGA 781 AAGCCTGTGA TGGTATCAAT GATTGTGGAG ACCAAAGTGA TGAACTGTGT TGTAAAGCAT 841 GCCAAGGCAA AGGCTTCCAT TGCAAATCGG GTGTTTGCAT TCCAAGCCAG TATCAATGCA 901 ATGGTGAGGT GGACTGCATT ACAGGGGAAG ATGAAGTTGG CTGTGCAGGC TTTGCATCTG 961 TGGCTCAAGA AGAAACAGAA ATTTTGACTG CTGACATGGA TGCAGAAAGA AGACGGATAA 1021 AATCATTATT ACCTAAACTA TCTTGTGGAG TTAAAAACAG AATGCACATT CGAAGGAAAC 1081 GAATTGTGGG AGGAAAGCGA GCACAACTGG GAAAAATGAA GCAAATCTCA TTGGATATTT 1141 TTAAAGGTCT CCACAGAGTT TATGCCATAT TGGAATTTTG TTGTATAATT CTCAAATACC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATTGGGTCC ACCCGTTTGT TTTTAAACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt