Construct: ORF TRCN0000473934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011244.1_s317c1
- Derived from:
- ccsbBroadEn_10247
- DNA Barcode:
- GTCTATCCATAATGCTTTAATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHPN1-AS1 (78998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78998 | RHPN1-AS1 | RHPN1 antisense RNA 1 (head... | NR_026785.1 | 8.7% | 1_290del;468_2030del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 248
- ORF end:
- 425
- ORF length:
- 177
- Sequence:
-
1 TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT 61 GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA TGTAATTCTC CTTGGAATTT 121 GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT TCAAAGTTTT 181 TTTCTTCCAT TTCAGGTGTC GTGAGGCTAG CATCGATTGA TCAACAAGTT TGTACAAAAA 241 AGTTGGCATG CCTGCTTTCT TCTCTCTGCC CGCGGAGCGG AGGCTCCAGG CCTGGCCCCA 301 GTCCGAGGCG CCTCTGTCGG TGTCGTCCTG CTTCCAAAAC CGGCCTCCAG AACCCGCCTC 361 CTTCCAGAAC CTCCGTCCAG AACCCGCCTC CCTCCAGAAC CTCCGTACAG AACCCACATC 421 CTTCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 481 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 541 TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCTATCCAT AATGCTTTAA TTACACGCGT 601 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt