Construct: ORF TRCN0000473949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009506.1_s317c1
- Derived from:
- ccsbBroadEn_15912
- DNA Barcode:
- AACGGCAACTTGAGGACCGCGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARFGAP1 (55738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281482.2 | 100% | 100% | |
| 2 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_018209.4 | 66.5% | 66.8% | 834_835ins239;971_1218del |
| 3 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_006723823.3 | 66.1% | 66.5% | 832_833ins245;965_1212del |
| 4 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_011528901.2 | 65.2% | 65.3% | 717_746del;864_865ins239;1001_1248del |
| 5 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XM_017027950.1 | 65.2% | 65.3% | 717_746del;864_865ins239;1001_1248del |
| 6 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_175609.3 | 64.8% | 65.1% | 717_746del;862_863ins245;995_1242del |
| 7 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281483.1 | 53.3% | 52.7% | (many diffs) |
| 8 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | NM_001281484.1 | 43.3% | 35.9% | (many diffs) |
| 9 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_001754329.2 | 29% | (many diffs) | |
| 10 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936574.2 | 28.1% | (many diffs) | |
| 11 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936576.2 | 26.2% | (many diffs) | |
| 12 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_936575.2 | 26% | (many diffs) | |
| 13 | human | 55738 | ARFGAP1 | ADP ribosylation factor GTP... | XR_001754330.2 | 25.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcccaaga accaggaagg ttcttaaaga agtcagggtg caggatgaga 121 acaacgtttg ttttgagtgt ggcgcgttca atcctcagtg ggtcagtgtg acctacggca 181 tctggatctg cctggagtgc tcggggagac accgcgggct tggggttcac ctcagctttg 241 tgcgctctgt tactatggac aagtggaagg acattgagct tgagaagatg aaagctggtg 301 ggaatgctaa gttccgagag ttcctggagt ctcaggagga ttacgatcct tgctggtcct 361 tgcaggagaa gtacaacagc agagccgcgg ccctctttag ggataaggtg gtcgctctgg 421 ccgaaggcag agagtggtct ctggagtcat cacctgccca gaactggacc ccacctcagc 481 ccaggacgct gccgtccatg gtgcaccgag tctctggcca gccgcagagt gtgaccgcct 541 cctcggacaa ggcttttgaa gactggctga atgatgacct cggctcctat caaggggccc 601 aggggaatcg ctacgtgggg tttgggaaca cgccaccgcc tcagaagaaa gaagatgact 661 tcctcaacaa cgccatgtcc tccctgtact cgggctggag cagcttcacc actggagcca 721 gccggtttgc ctcggcagcc aaggagggcg ctacaaagtt tggatcccaa gcgagtcaga 781 aggcgtccga gctgggccac agcctgaacg agaacgtcct caagcctGCG CAGGAGAAGG 841 TGAAGGAGGG AAAGATTTTT GATGATGTCT CCAGTGGGGT CTCTCAGTTG GCGTCCAAGT 901 GCCAGCGCCG TCTTTGCTGC CATCAGTCCC ACTGCTCTGC GGGCCATTTG GGGCGTGCAT 961 TTTGTCCTGT TTCCTGGCAT GAGGCGCTCT GCGGACAGAC GGGGAGGGAA GAGCAGGCCT 1021 CGCTCCTCCC CCCCAAGCAT GTGGTGGGAG CTCTTGAGGT CTGTGCACGA GGCTGTCCTC 1081 GCTGCCATGT CCCGCACACA CCTGGCACCG CTGCAGAGTG GCCGGGGCGT CTGTGTCTGT 1141 CCAGGGAGTC GGTAGTAAGG GATGGCGGGA CGTCACCACC TTTTTTTCGG GGAAAGCAGA 1201 GGGCCCCTTG GACAGCCCCT CGGAGGGCCA CAGTTATCAG AACAGCGGTC TGGACCACTT 1261 CCAAAACAGC AACATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACGGCAACT TGAGGACCGC 1441 GATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt