Construct: ORF TRCN0000473954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015004.1_s317c1
- Derived from:
- ccsbBroadEn_02377
- DNA Barcode:
- CCATGTGTATGGAGCAGTTCGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMP3 (10268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10268 | RAMP3 | receptor activity modifying... | NM_005856.3 | 100% | 100% | |
| 2 | human | 10268 | RAMP3 | receptor activity modifying... | XM_017011666.1 | 78.3% | 78.3% | 0_1ins96 |
| 3 | human | 10268 | RAMP3 | receptor activity modifying... | XM_006715631.3 | 57.1% | 57.1% | 58_390del |
| 4 | mouse | 56089 | Ramp3 | receptor (calcitonin) activ... | XM_006514761.3 | 67.1% | 69.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gactggagcg ctgcggcgcc cgcaacttct cccgttgctg ctgctgctct 121 gcggtgggtg tcccagagca ggcggctgca acgagacagg catgttggag aggctgcccc 181 tgtgtgGGAA GGCTTTCGCA GACATGATGG GCAAGGTGGA CGTCTGGAAG TGGTGCAACC 241 TGTCCGAGTT CATCGTGTAC TATGAGAGTT TCACCAACTG CACCGAGATG GAGGCCAATG 301 TCGTGGGCTG CTACTGGCCC AACCCCCTGG CCCAGGGCTT CATCACCGGC ATCCACAGGC 361 AGTTCTTCTC CAACTGCACC GTGGACAGGG TCCACTTGGA GGACCCCCCA GACGAGGTTC 421 TCATCCCGCT GATCGTTATA CCCGTCGTTC TGACTGTCGC CATGGCTGGC CTGGTGGTGT 481 GGCGCAGCAA ACGCACCGAC ACGCTGCTGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCATG 661 TGTATGGAGC AGTTCGGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt