Construct: ORF TRCN0000473964
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015408.1_s317c1
- Derived from:
- ccsbBroadEn_08833
- DNA Barcode:
- ATCCGCTTGTGACCGATTAGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELSPBP1 (64100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473964
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64100 | ELSPBP1 | epididymal sperm binding pr... | NM_022142.5 | 99.8% | 99.5% | 595G>A |
| 2 | human | 64100 | ELSPBP1 | epididymal sperm binding pr... | XM_017027130.1 | 83% | 82.8% | 1_135del;730G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ccgatggtcc agttacctgt tgggatggac aaccttcctt ctctattcct 121 atgagtcaag tggagggatg catgaggaat gtgtctttcc tttcacctac aagggatctg 181 tttacttcac ttgcacccat attcatagct taTCCCCTTG GTGTGCCACC AGAGCCGTGT 241 ACAACGGCCA GTGGAAGTAC TGCCAGAGTG AAGATTACCC ACGCTGTATC TTCCCTTTCA 301 TCTATCGAGG AAAGGCTTAT AACAGCTGCA TCTCCCAGGG CAGCTTCTTA GGCAGTCTGT 361 GGTGCTCAGT CACCTCTGTC TTCGATGAGA AACAGCAGTG GAAATTCTGT GAAACGAATG 421 AGTATGGGGG AAATTCTCTC AGGAAGCCCT GCATCTTCCC CTCCATCTAC AGAAATAATG 481 TGGTCTCTGA TTGCATGGAG GATGAAAGCA ACAAGCTCTG GTGCCCAACC ACAGAGAACA 541 TGGATAAGGA TGGAAAGTGG AGTTTCTGTG CCGACACCAG AATTTCCGCG TTGGTCCCTG 601 GCTTTCCTTG TCACTTTCCG TTCAACTATA AAAACAAGAA TTATTTTAAC TGCACTAACA 661 AAGGATCAAA GGAGAACCTT GTGTGGTGTG CAACTTCTTA CAACTACGAC CAAGACCACA 721 CCTGGGTGTA TTGCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCCGCTTGT GACCGATTAG 901 ACGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt