Construct: ORF TRCN0000473970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005345.1_s317c1
- Derived from:
- ccsbBroadEn_12313
- DNA Barcode:
- CTCCACGGCGGACGTAGCCCGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCLN (56926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56926 | NCLN | nicalin | NM_020170.4 | 97.9% | 98% | 1_33del;57G>T |
| 2 | human | 56926 | NCLN | nicalin | NM_001321463.2 | 97.8% | 97.8% | 1_33del;57G>T;1334_1335insGCA |
| 3 | mouse | 103425 | Ncln | nicalin | NM_134009.3 | 85.4% | 91.4% | (many diffs) |
| 4 | mouse | 103425 | Ncln | nicalin | XM_017313762.1 | 85.2% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaaggcgtct tgtctgcctc tcggcttcat cgtcttcctg cccgctgtgc 121 tgctgctggt ggcgccgccg ctgcctgccg ccgacgccgc gcacgagttc accgtgtacc 181 gcatgcagca gtacgacctg cagggccagc cctacggcac acggaatgca gtgctgaaca 241 cggaggcgcg cacgatggcg gcggaggtgc tgagccgccg ctgcgtgctc atgcggctac 301 tggacttctc ctacgagcag taccagaagg ccctgcggca gtcggcgggc gccgtggtca 361 tcatcctgcc cagggccatg gccgccgtgc cccaggacgt cgtccggcaa ttcatggaga 421 tcgagccgga gatgctggcc atggagaccg ccgtccccgt gtactttgcc gtggaggacg 481 aggccctgct gtctatctac aagcagaccc aggctgcctc cgcctcccag ggctccgcct 541 ctgctgctga agtactgctg cgcacggcca ctgccaacgg cttccagatg gtcaccagcg 601 gggtacagag caaggccgtg agtgactggc tgattgccag cgtggagggg cggctgacgg 661 ggctgggcgg agaggacctt cccaccatcg tcatcgtggc ccactacgac gcctttggag 721 tggccccctg gctgtcgctg ggcgcggact ccaacgggag cggcgtctct gtgctgctgg 781 agctggcacg cctcttctcc cggctctaca cctacaagcg cacgcacgcc gcctacaacc 841 tcctgttctt tgcgtctgga ggaggcaagt ttaactacca gggaaccaag cgctggctgg 901 aagacaacct ggaccacaca gactccagcc tgcttcagga caatgtggcc ttcgtgctgt 961 gcctggacac cgtgggccgg ggcagcagcc tgcacctgca cgtgtccaag ccgcctcggg 1021 agggcaccct gcagcacgcc ttcctgcggg agctggagac ggtggccgcg caccagttcc 1081 ctgaggtacg gttctccatg gtgcacaagc ggatcaacct ggcggaggac gtgctggcct 1141 gggagcacga gcgcttcgcc atccgccgac tgcccgcctt cacgctgtcc cacctggaga 1201 gccaccgtga cggccagcgc agcagcatca tggacgtgcg gtcccgggtg gattctaaga 1261 cccTGACCCG TAACACGAGG ATCATTGCAG AGGCCCTGAC TCGAGTCATC TACAACCTGA 1321 CAGAGAAGGG GACACCCCCA GACATGCCGG TGTTCACAGA GCAGATGCAG ATCCAGCAGG 1381 AGCAGCTGGA CTCGGTGATG GACTGGCTCA CCAACCAGCC GCGGGCCGCG CAGCTGGTGG 1441 ACAAGGACAG CACCTTCCTC AGCACGCTGG AGCACCACCT GAGCCGCTAC CTGAAGGACG 1501 TGAAGCAGCA CCACGTCAAG GCTGACAAGC GGGACCCAGA GTTTGTCTTC TACGACCAGC 1561 TGAAGCAAGT GATGAATGCG TACAGAGTCA AGCCGGCCGT CTTTGACCTG CTCCTGGCTG 1621 TTGGCATTGC TGCCTACCTC GGCATGGCCT ACGTGGCTGT CCAGCACTTC AGCCTCCTCT 1681 ACAAGACCGT CCAGAGGCTG CTCGTGAAGG CCAAGACACA GTGCCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGACTC CACGGCGGAC GTAGCCCGCT CACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t