Construct: ORF TRCN0000473979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014312.1_s317c1
- Derived from:
- ccsbBroadEn_13814
- DNA Barcode:
- ACGATACGAGTCGATGAAGAGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ARHGAP5 (394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473979
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 394 | ARHGAP5 | Rho GTPase activating prote... | NM_001030055.2 | 99.9% | 12.7% | (many diffs) |
| 2 | human | 394 | ARHGAP5 | Rho GTPase activating prote... | XM_005267635.4 | 99.9% | 12.7% | (many diffs) |
| 3 | human | 394 | ARHGAP5 | Rho GTPase activating prote... | XM_005267636.4 | 99.9% | 12.7% | (many diffs) |
| 4 | human | 394 | ARHGAP5 | Rho GTPase activating prote... | NM_001173.3 | 99.8% | 12.7% | (many diffs) |
| 5 | human | 394 | ARHGAP5 | Rho GTPase activating prote... | XM_017021288.1 | 99.8% | 12.7% | (many diffs) |
| 6 | mouse | 11855 | Arhgap5 | Rho GTPase activating prote... | NM_009706.2 | 93.4% | 12.4% | (many diffs) |
| 7 | mouse | 11855 | Arhgap5 | Rho GTPase activating prote... | XM_006515438.3 | 93.4% | 12.4% | (many diffs) |
| 8 | mouse | 11855 | Arhgap5 | Rho GTPase activating prote... | XM_011243989.1 | 93.4% | 12.4% | (many diffs) |
| 9 | mouse | 11855 | Arhgap5 | Rho GTPase activating prote... | XM_011243990.1 | 93.4% | 12.4% | (many diffs) |
| 10 | mouse | 11855 | Arhgap5 | Rho GTPase activating prote... | XM_006515439.2 | 93.3% | 12.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 648
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggcaaaa aacaaagagc ctcgtccccc atcctatacc atcagtatag 121 ttggactctc tgggactgaa aaagacaaag gtaactgtgg agttggaaag tcttgtttgt 181 gcaatagatt tgtacgctca aaagcagatg aatattatcc agagcatact tctgtgctta 241 gcaccattga ctttggagga cgagtagtaa acaatgatca ctttttgtac tggggtgaca 301 taatacaaaa tagtgaagat ggagtagaat gcaaaattca tgtcattgaa caaacagagt 361 tcattgatga ccagactttc ttgcctcatc ggagtacgaa tttgcaacca tatataaaac 421 gtgcagctgc atctaaattg cagtcagcag aaaaactaat gtacatttgc actgatcagc 481 taggcttaga acaagacttt gaacagaagc aaatgcctga agggaagctc aacgtagatg 541 gatttttatt atgcattgat gtaagtcaag gatgcaatag gaagtttgat gatcaactta 601 aatttgtgaa taaccttttt gtccagttat caaaatcaaa aaacctgtaa taatagcagc 661 aactaaatgt gatgaatgcg tggatcatta tcttagagaa gttcaggcat ttgcttcaaa 721 taaaaagaac cttcttgtag tggaaacatc agcacgattt aatgtcaaca ttgaaacatg 781 ttttactgca ctggtacaaa tgttggataa aactcgtagc aagcctaaaa ttattcccta 841 tttggatgct tataaaacac agagacaact tgttgtcaca gcaacagata agtttgaaaa 901 acttgtgcag actgtgagag attatcatgc aacttggaaa actgttagta ataaattaaa 961 aaatcatcct gattatgaag aatacatcaa cttagaggga acaagaaagg ccagaaatac 1021 attctcaaaa catatagaac aacttaaaca ggaacatata agaaaaagga gagaagagta 1081 tataaatact ttaccaagag cttttaacac tcttttgcca aatctagaag agattgaaca 1141 tttgaattgg tcagaagctt tgaagttaat ggaaaagaga gcagatttcc agttatgttt 1201 tgtggtgcta gaaaaaactc cttgggatga aactgaccat atagacaaaa ttaatgatag 1261 gcggattcca tttgacctcc tgagcacttt agaagctgaa aaagtctatc agaaccatgt 1321 acagcatctg atatccgaga agaggagggt ggaaatgaag gaaaaattca aaaagacttt 1381 ggaaaaaatt caattcattt caccagggca gccatgggag gaagttatgt gctttgttat 1441 ggaggatgaa gcctacaaat atatcactga ggctgatagc aaagaggtat atggtaggca 1501 tcagcgagaa atagttgaaa aagccaaaga agagtttcaa gaaatgcttt ttgagcattc 1561 tgaacttttt tatgatttag atcttaatgc aacacctagt tcagataaaa tgagtgaaat 1621 tcatacagtt ctgagtgaag aacctagata taaagcttta cagaaacttg cacctgatag 1681 ggaatccctt ctacttaagc atataggatt tgtttatcat cccactaaag aaacatgtct 1741 tagtggccaa aattgtacag acattaaagt ggagcagtta cttgctagta gtcttttaca 1801 gttggatcat ggccgcttaa gattatatca cgatagtacc aatatagata aagttaacct 1861 ttttatttta gggaaggatg gccttgccca agaactagca aatgagataa ggacacaatc 1921 cactgatgat gagtatgcct tagatggaaa aatttatgaa cttgatcttc ggccggttga 1981 tgccaaatcg ccttactttt tgagtcagtt atggactgcc gcctttaaac cacatgggtg 2041 cttctgtgta tttaattcca ttgagtcatt gagttttatt ggggaattta ttgggaaaat 2101 aagaactgaa gcttctcaga tcagaaaaga taaatacatg gctaatcttc catttacatt 2161 aattctggct aatcagagag attccattag taagaatcta ccaattctca ggcaccaagg 2221 gcagcagttg gcaaacaagt tgcaatgtcc ttttgtagat gtacctgctg gtacatatcc 2281 tcgtaaattt aatgaaaccc agataaagca agctctcaga ggagtattgg aatcagttaa 2341 acacaatttg gatgtggtga gcccaattcc tgccaataag gacttatcag aagctgactt 2401 gagaattgtc atgtgcgcca tgtgtggaga tccatttagt gtggatctta ttctttcacc 2461 cttccttgat tctcattctt gcagtgctgc tcaagctgga cagaataatt ccctaatgct 2521 tgataaaatc attggtgaaa aaaggaggcg aatacagatc acaatattat cgtaccactc 2581 ttcaattgga gtaagaaaag atgaactagt tcatgggtat atattagttt actctgcaaa 2641 acggaaagct tcgatgggaa tgcttcgagc atttctatca gaagttcaag acaccattcc 2701 tgtacagctg gtggcagtta ctgacagcca agcagatttt tttgaaaatg aggctatcaa 2761 agagttaatg actgaaggag aacacattgc aactgagatc actgctaaat ttacagcact 2821 gtattcttta tctcagtatc atcggcaaac tgaggtcttt actctgtttt ttagtgatgt 2881 tctagagaaa aaaaatatga tagaaaattc ttatttgtct gataatacaa gggaatcaac 2941 ccatcaaagt gaagatgttt ttctaccatc tcccagagac tgttttccct ataataacta 3001 ccctgattca gatgatgaca cagaagcacc acctccttat agtccaattg gggatgatgt 3061 acagttgctc ccaacaccta gtgaccgttc cagatataga ttagatttgg aaggaaatga 3121 gtatcctatt catagtaccc caaactgtca tgaccatgaa cgcaaccata aagtgcctcc 3181 acctattaaa cctaaaccag ttgtacctaa gacaaatgtg aaaaaactcg atccaaacct 3241 tttaaaaaca attgaagctg gtattggtaa aaatccaaga aagcagactt cccgggtgcc 3301 tttggcacat cctgaagata tggatccttc agataactat gcggaaccca ttgatacaat 3361 tttcaaacag aagggctatt ctgatgagat ttatgttgtc ccagatgata gtcaaaatcg 3421 tattaaaatt cgaaactcat ttgtaaataa cacccaagga gatgaagaaa atgggttttc 3481 tgatagaacc tcaaaaagtc atggggaacg gaggccttca aaatacaaat ataaatctaa 3541 aaccttgttt agtaaagcca agtcatacta tagaagaaca cattcagatg ccagtgatga 3601 tgaggctttc accacttcta aaacaaaaag aaaaggaaga catcgtggaa gtgaagaaga 3661 tccacttctt tctcctgttg aaacttggaa aggtggtatt gataatcctg caatcacttc 3721 tgaccaggag ttagatgata agaagatgaa gaagaaaacc cacaaagtga aagaagataa 3781 aaagcagaaa aagaaaacta agaacttcaa tccaccaaca cgtagaaatt gggaaagtaa 3841 ttactttggg atgcccctcc aggatctggt tacagctgag aagcccatac cactatttgt 3901 tgagaaatgt gtggaattta ttgaagatac agggttatgt accgaaggac tctaccgtgt 3961 cagcgggaat aaaactgacc aagacaatat tcaaaagcag tttgatcaag atcataatat 4021 caatctagtg tcaatggaag taacagtaaa tgctgtagct ggagccctta aagctttctt 4081 tgcagatctg ccagatcctt taattccata ttctcttcat ccagaactat tggaagcagc 4141 aaaaatcccg gataaaacag aacgtcttca tgCCTTGAAA GAAATTGTTA AGAAATTTCA 4201 TCCTGTAAAC TATGATGTAT TCAGATACGT GATAACACAT CTAAACAGGG TTAGTCAGCA 4261 ACATAAAATC AACCTAATGA CAGCAGACAA CTTATCCATC TGTTTTTGGC CAACCTTGAT 4321 GAGACCTGAT TTTGAAAATC GAGAGTTTCT GTCTACTACT AAGATTCATC AATCTGTTGT 4381 TGAAACATTC ATTCAGCAGT GTCAGTTTTT CTTTTACAAT GGAGAAATTG TAGAAACGAC 4441 AAACATTGTG GCTCCTCCAC CACCTTCAAA CCCAGGACAG TTGGTGGAAC CAATGGTGCC 4501 ACTTCAGTTG CCGCCACCAT TGCAACCTCA GCTGATACAA CCACAATTAC AAACGGATCC 4561 TCTTGGTATT ATATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 4621 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 4681 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CGATACGAGT CGATGAAGAG 4741 CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att