Construct: ORF TRCN0000473982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015225.1_s317c1
- Derived from:
- ccsbBroadEn_00466
- DNA Barcode:
- CTCACAATAAGTCCACACCGGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBEGF (1839)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1839 | HBEGF | heparin binding EGF like gr... | NM_001945.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctgctgccg tcggtggtgc tgaagctctt tctggctgca gttctctcgg 121 cactggtgac tggcgagagc ctggagcggc ttcggagagg gctagctgct ggaaccagca 181 acccggaccc tcccactgta tccacggacc agctgctacc cctaggaggc ggccgggacc 241 ggaaagtccg tgacttgcaa gaggcagatc tggacctttt gagagtcact ttatcctcca 301 agccacaagc actggccaCA CCAAACAAGG AGGAGCACGG GAAAAGAAAG AAGAAAGGCA 361 AGGGGCTAGG GAAGAAGAGG GACCCATGTC TTCGGAAATA CAAGGACTTC TGCATCCATG 421 GAGAATGCAA ATATGTGAAG GAGCTCCGGG CTCCCTCCTG CATCTGCCAC CCGGGTTACC 481 ATGGAGAGAG GTGTCATGGG CTGAGCCTCC CAGTGGAAAA TCGCTTATAT ACCTATGACC 541 ACACAACCAT CCTGGCCGTG GTGGCTGTGG TGCTGTCATC TGTCTGTCTG CTGGTCATCG 601 TGGGGCTTCT CATGTTTAGG TACCATAGGA GAGGAGGTTA TGATGTGGAA AATGAAGAGA 661 AAGTGAAGTT GGGCATGACT AATTCCCACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 721 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 781 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTCAC 841 AATAAGTCCA CACCGGGGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 901 tgaaagatt