Construct: ORF TRCN0000473984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007381.1_s317c1
- Derived from:
- ccsbBroadEn_06359
- DNA Barcode:
- CTGCACCGGGTTGCCTGAGCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEXA (3073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3073 | HEXA | hexosaminidase subunit alpha | NM_000520.6 | 99.8% | 99.8% | 9C>T;1306A>G;1518A>G |
| 2 | human | 3073 | HEXA | hexosaminidase subunit alpha | NM_001318825.2 | 97.7% | 97.7% | (many diffs) |
| 3 | human | 3073 | HEXA | hexosaminidase subunit alpha | NR_134869.2 | 72.4% | (many diffs) | |
| 4 | mouse | 15211 | Hexa | hexosaminidase A | XM_017313136.1 | 54.4% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac aagttccagg ctttggtttt cgctgctgct ggcggcagcg ttcgcaggac 121 gggcgacggc cctctggccc tggcctcaga acttccaaac ctccgaccag cgctacgtcc 181 tttacccgaa caactttcaa ttccagtacg atgtcagctc ggccgcgcag cccggctgct 241 cagtcctcga cgaggccttc cagcgctatc gtgacctgct tttcggttcc gggtcttggc 301 cccgtcctta cctcacaggg aaacggcata cactggagaa gaatgtgttg gttgtctctg 361 tagtcacacc tggatgtaac cagcttccta ctttggagtc agtggagaat tataccctga 421 ccataaatga tgaccagtgt ttactcctct ctgagactgt ctggggagct ctccgaggtc 481 tggagacttt tagccagctt gtttggaaat ctgctgaggg cacattcttt atcaacaaga 541 ctgagattga ggactttccc cgctttcctc accggggctt gctgttggat acatctcgcc 601 attacctgcc actctctagc atcctggaca ctctggatgt catggcgtac aataaattga 661 acgtgttcca ctggcatctg gtagatgatc cttccttccc atatgagagc ttcacttttc 721 cagagctcat gagaaagggg tcctacaacc ctgtcaccca catctacaca gcacaggatg 781 tgaaggaggt cattgaatac gcacggctcc ggggtatccg tgtgcttgca gagtttgaca 841 ctcctggcca cactttgtcc tggggaccag gtatccctgg attactgact ccttgctact 901 ctgggtctga gccctctggc acctttggac cagtgaatcc cagtctcaat aatacctatg 961 agttcatgag cacattcttc ttagaagtca gctctgtctt cccagatttt tatcttcatc 1021 ttggaggaga tgaggttgat ttcacctgct ggaagtccaa cccagagatc caggacttta 1081 tgaggaagaa aggcttcggt gaggacttca agcagctgga gtccttctac atccagacgc 1141 tgctggacat cGTCTCTTCT TATGGCAAGG GCTATGTGGT GTGGCAGGAG GTGTTTGATA 1201 ATAAAGTAAA GATTCAGCCA GACACAATCA TACAGGTGTG GCGAGAGGAT ATTCCAGTGA 1261 ACTATATGAA GGAGCTGGAA CTGGTCACCA AGGCCGGCTT CCGGGCCCTT CTCTCTGCCC 1321 CCTGGTACCT GAACCGTATA TCCTATGGCC CTGACTGGAA GGATTTCTAC GTAGTGGAAC 1381 CCCTGGCATT TGAAGGTACC CCTGAGCAGA AGGCTCTGGT GATTGGTGGA GAGGCTTGTA 1441 TGTGGGGAGA ATATGTGGAC AACACAAACC TGGTCCCCAG GCTCTGGCCC AGAGCAGGGG 1501 CTGTTGCCGA AAGGCTGTGG AGCAACAAGT TGACATCTGA CCTGACATTT GCCTATGAAC 1561 GTTTGTCACA CTTCCGCTGT GAGTTGCTGA GGCGAGGTGT CCAGGCCCAA CCCCTCAATG 1621 TAGGCTTCTG TGAGCAGGAG TTTGAACAGA CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 1801 GCACCGGGTT GCCTGAGCTT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1861 ttgtgaaaga tt