Construct: ORF TRCN0000474022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000686.1_s317c1
- Derived from:
- ccsbBroadEn_12081
- DNA Barcode:
- AGCAATCCTAAAAAGCCGACTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJB12 (54788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54788 | DNAJB12 | DnaJ heat shock protein fam... | NM_001002762.4 | 45.2% | 45.3% | 1_615del;903C>T |
| 2 | human | 54788 | DNAJB12 | DnaJ heat shock protein fam... | NM_001365081.2 | 45.2% | 45.3% | 1_615del;903C>T |
| 3 | human | 54788 | DNAJB12 | DnaJ heat shock protein fam... | NM_017626.6 | 45.2% | 45.3% | 1_615del;903C>T |
| 4 | human | 54788 | DNAJB12 | DnaJ heat shock protein fam... | NM_001365080.2 | 44.1% | 43.7% | (many diffs) |
| 5 | human | 54788 | DNAJB12 | DnaJ heat shock protein fam... | NR_157570.2 | 16.2% | 1_637del;925C>T;1148_3140del | |
| 6 | mouse | 56709 | Dnajb12 | DnaJ heat shock protein fam... | NM_019965.2 | 40.6% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctttggcggc ggcttccctt ctagtaacgt ccacgtctac agcaacggcc 121 gcatgcgcta tacctaccag caaaggcagg accgcaggga caaccagggt gatggcgggc 181 taggggtgtt tgtgcagctg atgcctatcc tcaTCCTGAT TCTCGTGTCA GCTCTCAGCC 241 AGCTCATGGT CTCCAGTCCA CCCTACAGTC TGAGTCCAAG ACCGTCCGTG GGCCACATCC 301 ACAGGCGAGT CACTGACCAC CTGGGTGTCG TCTACTATGT GGGAGACACT TTTTCCGAAG 361 AGTACACAGG CTCCAGCCTC AAAACAGTCG AGCGGAATGT GGAAGATGAT TATATCGCCA 421 ACCTCCGGAA CAACTGCTGG AAGGAGAAGC AGCAGAAGGA AGGCTTGCTG TACCGGGCAC 481 GCTACTTTGG CGACACAGAT ATGTACCACA GAGCACAGAA GATGGGCACC CCCAGCTGCA 541 GCCGACTGTC AGAGGTGCAG GCCTCCCTGC ATGGATACCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AAGCAATCCT AAAAAGCCGA CTGCTACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt