Construct: ORF TRCN0000474023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008435.1_s317c1
- Derived from:
- ccsbBroadEn_09803
- DNA Barcode:
- CTTAACCACATTTTTCTTTAGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTEX1D1 (200132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200132 | TCTEX1D1 | Tctex1 domain containing 1 | NM_152665.3 | 99.8% | 99.4% | 427C>A |
| 2 | human | 200132 | TCTEX1D1 | Tctex1 domain containing 1 | XM_017000566.1 | 99.8% | 99.4% | 427C>A |
| 3 | human | 200132 | TCTEX1D1 | Tctex1 domain containing 1 | XM_011540933.2 | 82% | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 606
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GATGATGTCA GACAATGCTA AAGGCAGAGC AGCTCATTCA TGGAAGAAAA 121 GAGGGAGTAT TTCTTCTCTA AGTAATCATG AATTTTGGCG AAAGGAAATT CATGGGCGCA 181 TCAAAGATTC TATGAGTACT GTGTCTTATA TGGAAGAACC CAGTCAGCGT GATGATATCT 241 CTCGCCTTAC AGTTCAGATG GAAAACACCT ATCAGTTGGG TCCTCCCAAA CATTTTCCTG 301 TGGTCACCGT CAATCATATT TTGAAAGATG TAGTAACCAG CTATCTACAA GTAGAAGAAT 361 ATGAACCAGA GCTCTGTAGA CAGATGACTA AAACCATTTC TGAGGTTATT AAAGCCCAGG 421 TCAAGGACTT GATGATTCCA CGGTATAAAC TAATTGTGAT TGTTCACATT GGACAACTGA 481 ACAGGCAGAG CATAATTATT GGAAGCAGAT GCCTCTGGGA TCCTAAAAGT GATACCTTTT 541 CATCTTATGT TTTCAGAAAT TCTTCTCTCT TCGCTCTTGC AAATGTCTAT GCAGTTTACC 601 TTGAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTTAACCAC ATTTTTCTTT AGAGAACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt