Construct: ORF TRCN0000474025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010668.1_s317c1
- Derived from:
- ccsbBroadEn_12344
- DNA Barcode:
- ATCTACGGCACTCGCATACAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRTM1 (57408)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57408 | LRTM1 | leucine rich repeats and tr... | NM_001304389.2 | 100% | 100% | |
| 2 | human | 57408 | LRTM1 | leucine rich repeats and tr... | NM_020678.4 | 77.9% | 77.9% | 1_228del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cttaaacttg tccaacaatt ccctttcaaa tctggcccct ggagctttcc 121 atgggcttca gcacttgcag gttttaaatc taacccagaa ttcactcctt tccctggaaa 181 gcagactttt ccattccctc cctcagctga gggagcttga tttgtcatca aacaacataa 241 gccaccttcc cacatccttg ggagagactt gggagaacct aactatactt gcggttcaac 301 aaaaccagct tcagcagctt gatcgagcgc tcctggaatC CATGCCCAGT GTGAGGCTTT 361 TACTTCTCAA GGACAACCTC TGGAAATGCA ATTGCCACTT GCTCGGTCTT AAACTCTGGC 421 TGGAGAAATT TGTCTATAAA GGGGGACTAA CAGACGGCAT CATCTGTGAA TCACCAGACA 481 CCTGGAAGGG AAAGGACCTC CTTAGGATCC CTCATGAGCT GTACCAGCCC TGCCCTCTTC 541 CTGCTCCTGA TCCAGTGTCC TCGCAGGCTC AGTGGCCCGG CTCTGCCCAC GGTGTGGTCC 601 TGAGGCCTCC TGAGAACCAC AACGCGGGGG AGCGAGAACT CTTGGAGTGC GAGCTCAAAC 661 CCAAGCCAAG GCCGGCCAAC CTGCGTCATG CCATTGCCAC TGTCATCATC ACTGGCGTTG 721 TGTGTGGGAT TGTGTGTCTC ATGATGTTGG CAGCTGCCAT CTATGGCTGC ACCTATGCGG 781 CAATCACAGC CCAGTACCAT GGGGGACCCT TGGCTCAAAC CAATGATCCT GGGAAGGTGG 841 AAGAAAAAGA GCGATTTGAC AGCTCACCAG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 1021 CTACGGCACT CGCATACAGT TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt