Construct: ORF TRCN0000474027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012030.1_s317c1
- Derived from:
- ccsbBroadEn_08509
- DNA Barcode:
- ATAGAATTAACATACCTAGTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLAH (55301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55301 | OLAH | oleoyl-ACP hydrolase | NM_001039702.3 | 99.8% | 99.6% | 13G>A |
| 2 | human | 55301 | OLAH | oleoyl-ACP hydrolase | XM_024448060.1 | 99.8% | 99.6% | 13G>A |
| 3 | human | 55301 | OLAH | oleoyl-ACP hydrolase | NM_018324.3 | 83.2% | 82.7% | 13G>A;164_322del |
| 4 | human | 55301 | OLAH | oleoyl-ACP hydrolase | XM_017016376.2 | 83.2% | 82.7% | 13G>A;164_322del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 861
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagaggaaac caacctaaga gaaccaggaa tgaaaacatt ttcaactgct 121 tatacaaaaa ccctgaggca acttttaagc tgatttgctt tccctggatg ggaggtggct 181 ccactcattt tgccaaatgg ggccaagata ctcatgattt gctggaagtg cactccttaa 241 ggcttcctgg aagagaaagc agagttgaag aacctcttga aaatgacatc tcccagttag 301 ttgatgaagt tgtttgtgct ctgcagccag tcatccagga taaaccattt gcattttttg 361 gccacagtat gggatccTAC ATTGCTTTTA GGACTGCACT AGGTCTAAAA GAAAACAATC 421 AACCAGAACC ATTGCATTTA TTTTTGTCAA GTGCAACTCC TGTACATTCA AAGGCCTGGC 481 ATCGCATTCC CAAAGATGAT GAATTGTCAG AAGAACAAAT AAGTCATTAC CTTATGGAAT 541 TTGGAGGCAC CCCCAAGCAT TTTGCTGAAG CCAAGGAATT TGTGAAACAA TGTAGTCCCA 601 TCATAAGGGC AGATCTGAAC ATTGTTAGAA GTTGCACCTC TAACGTACCA TCTAAGGCTG 661 TTCTTTCCTG TGACTTGACA TGTTTTGTTG GATCTGAAGA CATAGCAAAG GACATGGAAG 721 CCTGGAAAGA TGTAACCAGT GGAAATGCTA AAATTTACCA GCTTCCAGGG GGTCACTTTT 781 ATCTTCTGGA TCCTGCGAAC GAGAAATTAA TCAAGAACTA CATAATCAAG TGTCTAGAAG 841 TATCATCGAT ATCCAATTTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAG AATTAACATA 1021 CCTAGTCCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt