Construct: ORF TRCN0000474030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009904.1_s317c1
- Derived from:
- ccsbBroadEn_02173
- DNA Barcode:
- TTCTCCACCGCGACTTCCTGCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG5 (9474)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9474 | ATG5 | autophagy related 5 | NM_001286106.1 | 100% | 100% | |
| 2 | human | 9474 | ATG5 | autophagy related 5 | NM_004849.4 | 100% | 100% | |
| 3 | human | 9474 | ATG5 | autophagy related 5 | NM_001286108.1 | 77.4% | 60.9% | 477_478insGACA;639_640ins182 |
| 4 | human | 9474 | ATG5 | autophagy related 5 | NM_001286107.1 | 71.6% | 71.2% | 0_1ins233;2_3insG |
| 5 | human | 9474 | ATG5 | autophagy related 5 | XM_024446590.1 | 71.6% | 71.2% | 0_1ins233;2_3insG |
| 6 | human | 9474 | ATG5 | autophagy related 5 | NM_001286111.1 | 33% | 13.8% | 108_109ins370;273_274ins182 |
| 7 | human | 9474 | ATG5 | autophagy related 5 | NR_104402.1 | 24.6% | 1_58del;535_536ins95;789_2870del | |
| 8 | human | 9474 | ATG5 | autophagy related 5 | XR_001743757.2 | 24.6% | 1_322del;896_1033del;1286_3352del | |
| 9 | mouse | 11793 | Atg5 | autophagy related 5 | NM_053069.6 | 91.7% | 96.7% | (many diffs) |
| 10 | mouse | 11793 | Atg5 | autophagy related 5 | XM_011243108.2 | 91.7% | 96.7% | (many diffs) |
| 11 | mouse | 11793 | Atg5 | autophagy related 5 | XM_017313782.1 | 66% | 57.3% | (many diffs) |
| 12 | mouse | 11793 | Atg5 | autophagy related 5 | XM_017313783.1 | 65.4% | 58.5% | (many diffs) |
| 13 | mouse | 11793 | Atg5 | autophagy related 5 | NM_001314013.1 | 55.2% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 891
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agatgacaaa gatgtgcttc gagatgtgtg gtttggacga attccaactt 121 gtttcacgct atatcaggat gagataactg aaagggaagc agaaccatac tatttgcttt 181 tgccaagagt aagttatttg acgttggtaa ctgacaaagt gaaaaagcac tttcagaagg 241 ttatgagaca agaagacatt agtgagatat ggtttgaata tgaaggcaca ccactgaaat 301 ggcattatcc aattggtttg ctatttgatc ttcttgcatc aagttcagct cttccttgga 361 acatcacagt acattttaag agttttccag aaaaagaccT TCTGCACTGT CCATCTAAGG 421 ATGCAATTGA AGCTCATTTT ATGTCATGTA TGAAAGAAGC TGATGCTTTA AAACATAAAA 481 GTCAAGTAAT CAATGAAATG CAGAAAAAAG ATCACAAGCA ACTCTGGATG GGATTGCAAA 541 ATGACAGATT TGACCAGTTT TGGGCCATCA ATCGGAAACT CATGGAATAT CCTGCAGAAG 601 AAAATGGATT TCGTTATATC CCCTTTAGAA TATATCAGAC AACGACTGAA AGACCTTTCA 661 TTCAGAAGCT GTTTCGTCCT GTGGCTGCAG ATGGACAGTT GCACACACTA GGAGATCTCC 721 TCAAAGAAGT TTGTCCTTCT GCTATTGATC CTGAAGATGG GGAAAAAAAG AATCAAGTGA 781 TGATTCATGG AATTGAGCCA ATGTTGGAAA CACCTCTGCA GTGGCTGAGT GAACATCTGA 841 GCTACCCGGA TAATTTTCTT CATATTAGTA TCATCCCACA GCCAACAGAT TGCCCAACTT 901 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 961 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1021 CTTGTGGAAA GGACGATTCT CCACCGCGAC TTCCTGCAGG ACGCGTTAAG TCgacaatca 1081 acctctggat tacaaaattt gtgaaagatt