Construct: ORF TRCN0000474037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009245.1_s317c1
- Derived from:
- ccsbBroadEn_00863
- DNA Barcode:
- TGAAGACTCCGGAACGTCTCACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL15 (3600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3600 | IL15 | interleukin 15 | NM_000585.5 | 100% | 100% | |
| 2 | human | 3600 | IL15 | interleukin 15 | NM_172175.3 | 79.1% | 75.5% | (many diffs) |
| 3 | human | 3600 | IL15 | interleukin 15 | NR_037840.3 | 19.5% | 1_863del;1350_2491del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 576
- ORF length:
- 486
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGAATTTC GAAACCACAT TTGAGAAGTA 121 TTTCCATCCA GTGCTACTTG TGTTTACTTC TAAACAGTCA TTTTCTAACT GAAGCTGGCA 181 TTCATGTCTT CATTTTGGGC TGTTTCAGTG CAGGGCTTCC TAAAACAGAA GCCAACTGGG 241 TGAATGTAAT AAGTGATTTG AAAAAAATTG AAGATCTTAT TCAATCTATG CATATTGATG 301 CTACTTTATA TACGGAAAGT GATGTTCACC CCAGTTGCAA AGTAACAGCA ATGAAGTGCT 361 TTCTCTTGGA GTTACAAGTT ATTTCACTTG AGTCCGGAGA TGCAAGTATT CATGATACAG 421 TAGAAAATCT GATCATCCTA GCAAACAACA GTTTGTCTTC TAATGGGAAT GTAACAGAAT 481 CTGGATGCAA AGAATGTGAG GAACTGGAGG AAAAAAATAT TAAAGAATTT TTGCAGAGTT 541 TTGTACATAT TGTCCAAATG TTCATCAACA CTTCTTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATGAAGACTC CGGAACGTCT CACGGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt