Construct: ORF TRCN0000474042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011354.1_s317c1
- Derived from:
- ccsbBroadEn_13837
- DNA Barcode:
- ACATCCTCAACGCGACCGGTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRABP2 (1382)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1382 | CRABP2 | cellular retinoic acid bind... | NM_001199723.1 | 99.5% | 98.5% | 404A>C;412G>A |
| 2 | human | 1382 | CRABP2 | cellular retinoic acid bind... | NM_001878.4 | 99.5% | 98.5% | 404A>C;412G>A |
| 3 | mouse | 12904 | Crabp2 | cellular retinoic acid bind... | NM_007759.2 | 88.8% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caacttctct ggcaactgga aaaTCATCCG ATCGGAAAAC TTCGAGGAAT 121 TGCTCAAAGT GCTGGGGGTG AATGTGATGC TGAGGAAGAT TGCTGTGGCT GCAGCGTCCA 181 AGCCAGCAGT GGAGATCAAA CAGGAGGGAG ACACTTTCTA CATCAAAACC TCCACCACCG 241 TGCGCACCAC AGAGATTAAC TTCAAGGTTG GGGAGGAGTT TGAGGAGCAG ACTGTGGATG 301 GGAGGCCCTG TAAGAGCCTG GTGAAATGGG AGAGTGAGAA TAAAATGGTC TGTGAGCAGA 361 AGCTCCTGAA GGGAGAGGGC CCCAAGACCT CGTGGACCAG AGAACTGACC AACGATGGGG 421 AACTGATCCT GACCATGACG GCGGATGACG TTGTGTGCAC CAGGGTCTCC GTCCGAAAGT 481 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGAACATC CTCAACGCGA CCGGTCTACA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt