Construct: ORF TRCN0000474043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000784.1_s317c1
- Derived from:
- ccsbBroadEn_02620
- DNA Barcode:
- TTCCTGCCAAGCTTAGGTAATTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPP14 (11102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11102 | RPP14 | ribonuclease P/MRP subunit p14 | NM_001098783.2 | 100% | 100% | |
| 2 | human | 11102 | RPP14 | ribonuclease P/MRP subunit p14 | NM_007042.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 438
- ORF length:
- 372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgcccctgct gccacatatg aaagagtagt ttacaaaaac ccttccgagt 121 acCACTACAT GAAAGTCTGC CTAGAATTTC AAGATTGTGG AGTTGGACTG AATGCTGCAC 181 AGTTCAAACA GCTGCTTATT TCGGCTGTGA AGGACCTGTT TGGGGAGGTT GATGCCGCCT 241 TACCTTTGGA CATCCTAACC TATGAAGAGA AGACCTTGTC AGCCATCTTG AGAATATGTA 301 GCAGTGGTCT TGTCAAATTG TGGAGCTCTT TGACCCTGTT AGGATCCTAT AAAGGCAAAA 361 AATGTGCTTT CCGGGTGATT CAGGTTTCTC CATTTCTTCT TGCATTATCT GGTAATAGTA 421 GGGAACTAGT ATTGGATTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCCTGC CAAGCTTAGG 601 TAATTCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt