Construct: ORF TRCN0000474046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008599.1_s317c1
- Derived from:
- ccsbBroadEn_13510
- DNA Barcode:
- GCAAGGTCACAATTATATGTAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MILR1 (284021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_017024487.1 | 95.8% | 95.6% | 0_1ins36;2T>A |
| 2 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001085423.2 | 86.5% | 86.5% | 1_138del |
| 3 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001369493.1 | 86.5% | 86.5% | 1_138del |
| 4 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001291317.2 | 81% | 78.1% | (many diffs) |
| 5 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450706.1 | 77.1% | 77.1% | 1_264del |
| 6 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450707.1 | 77.1% | 77.1% | 1_264del |
| 7 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450708.1 | 77.1% | 77.1% | 1_264del |
| 8 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XM_024450709.1 | 77.1% | 77.1% | 1_264del |
| 9 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XR_002957990.1 | 62.7% | 1_285del;1177_1419del | |
| 10 | human | 284021 | MILR1 | mast cell immunoglobulin li... | XR_002957989.1 | 60.4% | 1_285del;1177_1475del | |
| 11 | human | 284021 | MILR1 | mast cell immunoglobulin li... | NM_001291316.2 | 58.8% | 58.8% | 1_138del;366_367ins285 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 960
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagggtcaa aatgtatcta tgttttgttc ccataagaac aaatcactgc 121 agatcaccta ttcattgttt cgacgtaaga cacacctggg aacccaggat ggaaaaggtg 181 aacctgcgat ttttaaccta agcatcacag aagcccatga atcaggcccc tacaaatgca 241 aagcccaagt taccagctgt tcaaaataca gtcgtgactt cagcttcacg attgtcgacc 301 cggtgacttc cccagtgctg aacattatgg tcattcaaac agaaacagac cgacatataa 361 cattacattg cctctcagtc aatggctcgc tgcccatcaa ttacactttc tttgaaaacc 421 atgttgccat atcaccagct atttccaagt atgacaggga gcctgctgaa tttaacttaa 481 ccaagaagaa tcctggagaa GAGGAAGAGT ATAGGTGTGA AGCTAAAAAC AGATTGCCTA 541 ACTATGCAAC ATACAGTCAC CCTGTCACCA TGCCCTCAAC AGGCGGAGAC AGCTGTCCTT 601 TCTGTCTGAA GCTACTACTT CCAGGGTTAT TACTGTTGCT GGTGGTGATA ATCCTAATTC 661 TGGCTTTTTG GGTACTGCCC AAATACAAAA CAAGAAAAGC TATGAGAAAT AATGTGCCCA 721 GGGACCGTGG AGACACAGCC ATGGAAGTTG GAATCTATGC AAATATCCTT GAAAAACAAG 781 CAAAGGAGGA ATCTGTGCCA GAAGTGGGAT CCAGGCCGTG TGTTTCCACA GCCCAAGATG 841 AGGCCAAACA CTCCCAGGAG CTACAGTATG CCACCCCCGT GTTCCAGGAG GTGGCACCAA 901 GAGAGCAAGA AGCCTGTGAT TCTTATAAAT CTGGATATGT CTATTCTGAA CTCAACTTCT 961 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAGCAAG GTCACAATTA TATGTAAGTA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt