Construct: ORF TRCN0000474057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008826.1_s317c1
- Derived from:
- ccsbBroadEn_14031
- DNA Barcode:
- ATAATTTGGATTAACGGATTACTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOMM70 (9868)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9868 | TOMM70 | translocase of outer mitoch... | NM_014820.5 | 99.7% | 99.1% | 70_72delGGC;1813delC |
| 2 | mouse | 28185 | Tomm70a | translocase of outer mitoch... | NM_138599.5 | 88.7% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1887
- ORF length:
- 1821
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcctctaaa cctgtggagg cagcggtggt cgcagccgct gtaccgagct 121 ccgggagtgg ggtgggcggc gggactgcgg gcccgggcac gggggggctg ccgcgatggc 181 agctggctct ggcggtcggg gcacccctgc tgctgggcgc gggtgccata tacctgtgga 241 gtcggcagca acggcgccgg gaggccagag gccggggcga cgccagcggc ctgaagcgca 301 acagcgaacg gaagaccccg gagggcaggg ccagtccggc cccgggcagc ggacaccctg 361 aaggtcccgg tgctcacttg gacatgaact ctcttgatag agcccaagca gccaagaata 421 aaggcaataa atattttaaa gcaggaaaat atgaacaagc tattcagtgc tatactgagg 481 ctattagctt gtgccctaca gagaagaatg ttgacctttc tacattttat caaaacagag 541 ctgctgcctt tgaacagttg caaaaatgga aagaagtggc acaagactgt acaaaagctg 601 ttgaacttaa tcccaaatat gtgaaagctc tctttagacg tgcaaaagcc catgagaagc 661 tagacaataa gaaggaatgt ttagaagatg tcactgctgt gtgtatatta gaagggttcc 721 aaaatcaaca aagcatgctg ttagccgata aagttcttaa actccttgga aaagagaaag 781 ccaaagaaaa atataagaat cgtgaacctc tgatgccatc tccacagttt atcaaatctt 841 acttcagttc tttcacggat gatatcattt cccagcccat gcttaaagga gagaaatctg 901 atgaagataa agacaaggaa ggggaggctt tagaagtgaa agaaaattct ggatacttaa 961 aggccaaaca gtatatggaa gaagaaaact acgataaaat cataagtgaa tgctcaaaag 1021 aaatagatgc tgaaggcaaa tacatggcag aagcattgct actacgagct accttctacc 1081 tgcttattgg caatgccaat gcagccaaac cagatttaga taaagtcatc agtttgaaag 1141 aagctaatgt gaagcttcga gcaaatgctc tcatcaaaag aggcagcatg tacatgcaac 1201 agcagcagcc tttgctgtcc actcaagatt ttaacatggc tgctgacatc gatcctcaga 1261 atgcagatgt ttatcaccac cgaggacagc tgaaaatact ccttgatcaa gttgaagaag 1321 cagtggcaga ttttgatgaa tgtattaggt taagacctga gtctgctctg gcacaagcac 1381 agaaatgttt tgcattgtac cgccaggcat atacgggaaa caactcttca caaatccaag 1441 cagctatgaa aggttttgaa gaggTCATAA AGAAATTTCC AAGGTGTGCC GAAGGCTATG 1501 CACTATACGC CCAGGCATTA ACAGATCAAC AACAGTTTGG TAAAGCTGAT GAAATGTATG 1561 ATAAATGTAT TGATTTGGAA CCAGATAATG CTACAACATA TGTTCATAAA GGTTTACTTC 1621 AACTTCAGTG GAAGCAAGAT CTGGATAGAG GTTTGGAACT TATCAGCAAG GCTATTGAAA 1681 TTGACAATAA ATGTGATTTT GCCTATGAAA CCATGGGAAC TATTGAAGTA CAAAGAGGAA 1741 ACATGGAGAA AGCCATTGAC ATGTTCAACA AAGCTATTAA CCTGGCCAAA TCGGAAATGG 1801 AGATGGCCCA TCTGTATTCA CTTTGCGATG CCGCCCATGC CCAGACAGAA GTTGCAAAGA 1861 AATACGGATT AAAACACCAA CATTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG 1921 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 1981 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AATAATTTGG 2041 ATTAACGGAT TACTTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 2101 agatt