Construct: ORF TRCN0000474063
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009768.1_s317c1
- Derived from:
- ccsbBroadEn_03451
- DNA Barcode:
- GGCGCCGAAGGCGATCCGCGCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35F2 (54733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474063
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54733 | SLC35F2 | solute carrier family 35 me... | NM_017515.5 | 98.6% | 98.6% | 1108_1122del |
| 2 | mouse | 72022 | Slc35f2 | solute carrier family 35, m... | NM_028060.3 | 83.8% | 88.2% | (many diffs) |
| 3 | mouse | 72022 | Slc35f2 | solute carrier family 35, m... | XM_006511461.3 | 81% | 85.3% | (many diffs) |
| 4 | mouse | 72022 | Slc35f2 | solute carrier family 35, m... | XM_006511462.3 | 74.4% | 80.2% | (many diffs) |
| 5 | mouse | 72022 | Slc35f2 | solute carrier family 35, m... | XM_006511460.2 | 72.6% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1173
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcagactcg ccagcgggcc ccggcgcccc agagcccctc gcggagggag 121 cggcggccga gttctccagc ctgctgcgca ggataaaagg caaactcttc acctggaata 181 ttttgaaaac aattgccctg ggtcagatgt tgtccttgtg tatatgtggg acagccatca 241 ccagccagta tttggcagaa agatacaaag tgaacacccc catgcttcag agctttatca 301 attattgctt gctgttccta atttatacag tgatgctggc atttcgatca ggcagtgata 361 accttttagt aatcttgaaa agaaaatggt ggaagtacat cctgctggga ctagcagatg 421 tggaagctaa ttatgtgatc gtcagagcct accagtacac aactctaacc agtgtccagc 481 ttttggattg ctttgggatt cctgtgttga tggctctgtc atggtttatt cttcatgcaa 541 gatacagagt gatccacttc atcgccgtgg ctgtctgtct gttgggtgta ggaaccatgg 601 ttggtgcaga catactagca gggagggaag acaattcagg gagtgatgta ttgattggtg 661 acatcttggt ccttcttGGG GCTTCCCTCT ATGCCATTTC AAATGTTTGT GAGGAATACA 721 TCGTGAAGAA GCTGAGCAGA CAGGAGTTTT TAGGAATGGT GGGCCTGTTT GGAACAATTA 781 TCAGTGGTAT ACAGCTATTG ATTGTGGAAT ATAAGGATAT TGCCAGCATT CATTGGGACT 841 GGAAAATTGC CCTGCTGTTC GTGGCATTTG CCCTGTGTAT GTTTTGCCTG TACAGCTTCA 901 TGCCATTGGT GATTAAAGTC ACTAGTGCCA CTTCCGTCAA CCTGGGCATC CTGACAGCGG 961 ACCTCTACAG CCTTTTTGTT GGACTCTTTC TGTTTGGCTA TAAGTTTTCA GGACTCTACA 1021 TCCTGTCCTT CACTGTCATC ATGGTGGGGT TTATCCTGTA CTGCTCCACC CCTACTCGCA 1081 CGGCCGAGCC GGCTGAAAGC AGCGTGCCTC CAGTCACCAG CATTGGGATT GACAACCTGG 1141 GGCTGAAGCT GGAGGAGAAC CTCCAGGAGA CCCACTCTGC TGTCTTGTAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAGGCGCCG AAGGCGATCC GCGCGTTACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt