Construct: ORF TRCN0000474069

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010951.1_s317c1
Derived from:
ccsbBroadEn_15535
DNA Barcode:
GGCGGAAAACGGTTCCTCTTGACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRH1 (5554)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474069
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5555 PRH2 proline rich protein HaeIII... NM_001110213.1 98.5% 98.7% (many diffs)
2 human 5554 PRH1 proline rich protein HaeIII... NM_001291314.2 88.5% 88.2% 124C>A;214_276del
3 human 5554 PRH1 proline rich protein HaeIII... NM_001291315.2 78.7% 73% (many diffs)
4 human 100533464 PRH1-PRR4 PRH1-PRR4 readthrough NR_037918.2 30.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
567
ORF length:
498
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcttctgatt ctgctgtcag tggccctgct ggccttcagc tcagctcagg
121atttaaatga agatgtcagc caggaagatg ttcccctcgt aatatcagat ggaggagact
181cTGAGCAGTT CATAGATGAG GAGCGTCAGG GACCACCTTT GGGAGGACAG CAATCTCAAC
241CCTCTGCTGG TGATGGGAAC CAGGATGATG GCCCTCAGCA GGGACCACCC CAACAAGGAG
301GCCAGCAGCA ACAAGGTCCA CCACCTCCTC AGGGAAAGCC ACAAGGACCA CCCCAACAGG
361GAGGCCATCC CCCTCCTCCT CAAGGAAGGC CACAAGGACC ACCCCAACAG GGAGGCCATC
421CCCGTCCTCC TCGAGGAAGG CCACAAGGAC CACCCCAACA GGGAGGCCAT CAGCAAGGTC
481CTCCCCCACC TCCTCCTGGA AAGCCCCAGG GACCACCTCC CCAAGGGGGC CGCCCACAAG
541GACCTCCACA GGGGCAGTCT CCTCAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
601GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
661AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCGGAAA
721ACGGTTCCTC TTGACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
781aagatt

Download FASTA (ORF) (Full)

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