Construct: ORF TRCN0000474082
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006586.1_s317c1
- Derived from:
- ccsbBroadEn_08128
- DNA Barcode:
- CACATAAACTATGTGTCGTAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG5 (29880)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474082
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29880 | ALG5 | ALG5 dolichyl-phosphate bet... | NM_013338.5 | 99.8% | 100% | 6T>C |
| 2 | human | 29880 | ALG5 | ALG5 dolichyl-phosphate bet... | NM_001142364.1 | 90.6% | 90.7% | 6T>C;282_283ins90 |
| 3 | human | 29880 | ALG5 | ALG5 dolichyl-phosphate bet... | XM_017020552.1 | 80.5% | 72.6% | 0_1ins142;96_97ins47 |
| 4 | human | 29880 | ALG5 | ALG5 dolichyl-phosphate bet... | XR_001749540.2 | 76.6% | (many diffs) | |
| 5 | mouse | 66248 | Alg5 | asparagine-linked glycosyla... | NM_025442.3 | 86.1% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccgcttctg ttgcagctgg cggtgctcgg cgcggcgctg gcggccgcag 121 ccctcgtact gatttccatc gttgcattta caactgctac aaaaatgcca gcactccatc 181 gacatgaaga agagaaattc ttcttaaatg ccaaaggcca gaaagaaact ttacccagca 241 tatgggactc acctaccaaa caactttctg tcgttgtgcc ttcatacaat gaagaaaaac 301 ggttgcctgt gatgatggat gaagctctga gctatctaga gaagagacag aaacgagatc 361 ctgcgttcac ttatgaagtg atagtagttg atgatggcag taaagatcag acctcaaagg 421 tagcttttaa atattgccag aaatatggaa gtgacaaagt acgtgtgata accctggtga 481 agaatcgtgg aaaaggtgga gcgattagaa tgggtatatt cagttctcga ggagaaaaga 541 tccttatggc agatgctgat ggagccacaa agtttccaga tgttgagaaa ttagaaaagg 601 ggctaaatga tctacagcct tggcctaatc aaatggctat agcatgtgga tctcgagctc 661 atttagaaaa agaatcaatt gctcagcgtt cttacttccg tactcttctc atgtatgggt 721 tccactttct ggtgtggttc ctttgtgtca aaggaatcag ggacacacag tgtgggttca 781 aattatttac tcgagaagca gcttcacgga cgttttcatc tctacacgtt gaacgatggg 841 CATTTGATGT AGAACTACTG TACATAGCAC AGTTCTTTAA AATTCCAATA GCAGAAATTG 901 CTGTCAACTG GACAGAAATT GAAGGTTCTA AATTAGTTCC ATTCTGGAGC TGGCTACAAA 961 TGGGTAAAGA CCTACTTTTT ATACGACTTC GATATTTGAC TGGTGCCTGG AGGCTTGAGC 1021 AAACTCGGAA AATGAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACATAA ACTATGTGTC 1201 GTAATTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt