Construct: ORF TRCN0000474092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014853.1_s317c1
- Derived from:
- ccsbBroadEn_03765
- DNA Barcode:
- CTTGTTATAGCTAGCCTAGTGGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DTWD1 (56986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56986 | DTWD1 | DTW domain containing 1 | NM_001144955.2 | 99.8% | 48% | 418_419insT |
| 2 | human | 56986 | DTWD1 | DTW domain containing 1 | NM_020234.6 | 99.8% | 48% | 418_419insT |
| 3 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022419.1 | 99.8% | 48% | 418_419insT |
| 4 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022420.1 | 99.8% | 48% | 418_419insT |
| 5 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022421.1 | 99.8% | 48% | 418_419insT |
| 6 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022422.1 | 99.8% | 48% | 418_419insT |
| 7 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022423.2 | 79% | 55% | (many diffs) |
| 8 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022424.1 | 79% | 55% | (many diffs) |
| 9 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_011521815.2 | 74% | 64.6% | (many diffs) |
| 10 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022425.1 | 74% | 64.6% | (many diffs) |
| 11 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022426.1 | 74% | 64.6% | (many diffs) |
| 12 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022427.1 | 71.1% | 19% | 0_1ins261;2T>A;157_158insT |
| 13 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022428.1 | 71.1% | 19% | 0_1ins261;2T>A;157_158insT |
| 14 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022429.1 | 71.1% | 19% | 0_1ins261;2T>A;157_158insT |
| 15 | human | 56986 | DTWD1 | DTW domain containing 1 | XM_017022430.2 | 71.1% | 19% | 0_1ins261;2T>A;157_158insT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctcaatcca cctatatttc tcaaacgaag tgaagaaaat agttcaaaat 121 ttgtggaaac aaaacagtca caaactactt ccatagcttc agaagatccc cttcaaaact 181 tatgtttagc atctcaagaa gttcttcaaa aagctcagca aagtgggaga tcaaaatgtc 241 tcaaatgtgg tggttccaga atgttctact gctatacatg ttatgttcca gttgaaaatg 301 tacctattga acagattcca cttgtgaagc ttccattgaa gattgacatc attaaacatc 361 caaatgaaac agatggcaaa agtactgcta tacatgcaaa actcttagca cctgaatttg 421 taaacattta cacgtatCCG TGTATTCCAG AATATGAAGA AAAGGACCAT GAAGTTGCAC 481 TCATTTTTTC CTGGACCTCA GTCTATCTCA ATAAAAGATA TTTCTTTTCA TCTGCAAAAA 541 AGGATTCAAA ATAATGTTAG AGGCAAAAAT GATGACCCTG ACAAGCCATC TTTTAAACGC 601 AAAAGAACTG AAGAACAAGA GTTCTGTGAT TTGAATGACA GCAAGTGCAA AGGCACAACA 661 CTGAAAAAAA TTATATTTAT AGATAGCACC TGGAACCAAA CAAACAAAAT ATTCACTGAT 721 GAGCGACTTC AAGGGTTGTT ACAAGTTGAG TTGAAAACAA GAAAAACTTG CTTTTGGCGC 781 CATCAAAAAG GAAAGCCAGA TACTTTCCTT TCTACAATTG AAGCCATTTA CTACTTTCTG 841 GTAGACTACC ATACTGATAT ATTAAAAGAG AAATACAGAG GGCAATATGA CAATCTTTTA 901 TTTTTCTATT CTTTTATGTA CCAGTTGATA AAGAATGCCA AATGCTCTGG AGATAAGGAA 961 ACAGGAAAAC TTACACATAA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 1021 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 1081 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTGTT ATAGCTAGCC 1141 TAGTGGTTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt