Construct: ORF TRCN0000474094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006084.2_s317c1
- Derived from:
- ccsbBroadEn_15114
- DNA Barcode:
- CACGGGTCGCCTACTCATACCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK6 (56924)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 106821730 | BUB1B-PAK6 | BUB1B-PAK6 readthrough | NM_001128628.2 | 100% | 100% | |
| 2 | human | 106821730 | BUB1B-PAK6 | BUB1B-PAK6 readthrough | NM_001128629.3 | 100% | 100% | |
| 3 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_001276717.1 | 100% | 100% | |
| 4 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_020168.5 | 100% | 100% | |
| 5 | human | 56924 | PAK6 | p21 (RAC1) activated kinase 6 | NM_001276718.2 | 93.3% | 93.3% | 1741_1742ins135 |
| 6 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | NM_001033254.3 | 88.7% | 92.5% | (many diffs) |
| 7 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | NM_001145854.1 | 88.7% | 92.5% | (many diffs) |
| 8 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XM_011239419.1 | 88.7% | 92.5% | (many diffs) |
| 9 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XM_011239420.2 | 88.7% | 92.5% | (many diffs) |
| 10 | mouse | 214230 | Pak6 | p21 protein (Cdc42/Rac)-act... | XR_866265.2 | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2109
- ORF length:
- 2043
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccgcaagaaa aagaagaaac gccctgagat ctcagcgcca cagaacttcc 121 agcaccgtgt ccacacctcc ttcgacccca aagaaggcaa gtttgtgggc ctccccccac 181 aatggcagaa catcctggac acactgcggc gccccaagcc cgtggtggac ccttcgcgaa 241 tcacacgggt gcagctccag cccatgaaga cagtggtgcg gggcagcgcg atgcctgtgg 301 atggctacat ctcggggctg ctcaacgaca tccagaagtt gtcagtcatc agctccaaca 361 ccctgcgtgg ccgcagcccc accagccggc ggcgggcaca gtccctgggg ctgctggggg 421 atgagcactg ggccaccgac ccagacatgt acctccagag cccccagtct gagcgcactg 481 acccccacgg cctctacctc agctgcaacg ggggcacacc agcaggccac aagcagatgc 541 cgtggcccga gccacagagc ccacgggtcc tgcccaatgg gctggctgca aaggcacagt 601 ccctgggccc cgccgagttt cagggtgcct cgcagcgctg tctgcagctg ggtgcctgcc 661 tgcagagctc cccaccagga gcctcgcccc ccacgggcac caataggcat ggaatgaagg 721 ctgccaagca tggctctgag gaggcccggc cacagtcctg cctggtgggc tcagccacag 781 gcaggccagg tggggaaggc agccctagcc ctaagacccg ggagagcagc ctgaagcgca 841 ggctattccg aagcatgttc ctgtccactg ctgccacagc ccctccaagc agcagcaagc 901 caggccctcc accacagagc aagcccaact cctctttccg accgccgcag aaagacaacc 961 ccccaagcct ggtggccaag gcccagtcct tgccctcgga ccagccggtg gggaccttca 1021 gccctctgac cacttcggat accagcagcc cccagaagtc cctccgcaca gccccggcca 1081 caggccagct tccaggccgg tcttccccag cgggatcccc ccgcacctgg cacgcccaga 1141 tcagcaccag caacctgtac ctgccccagg accccacggt tgccaagggt gccctggctg 1201 gtgaggacac aggtgttgtg acacatgagc agttcaaggc tgcgctcagg atggtggtgg 1261 accagggtga cccccggctg ctgctggaca gctacgtgaa gattggcgag ggctccaccg 1321 gcatcgtctg cttggcccgg gagaagcact cgggccgcca ggtggccgtc aagatgatgg 1381 acctcaggaa gcagcagcgc agggagctgc tcttcaacga ggtggtgatc atgcgggact 1441 accagcactt caacgtggtg gagatgtaca agagctacct ggtgggcgag gagctgtggg 1501 tgctcatgga gttcctgcag ggaggagccc tcacagacat cgtctcccaa gtcaggctga 1561 atgaggagca gattgccact gtgtgtgagg ctgtgctgca ggccctggcc tacctgcatg 1621 ctcagggtgt catccaccgg gacatcaaga gtgactccaT CCTGCTGACC CTCGATGGCA 1681 GGGTGAAGCT CTCGGACTTC GGATTCTGTG CTCAGATCAG CAAAGACGTC CCTAAGAGGA 1741 AGTCCCTGGT GGGAACCCCC TACTGGATGG CTCCTGAAGT GATCTCCAGG TCTTTGTATG 1801 CCACTGAGGT GGATATCTGG TCTCTGGGCA TCATGGTGAT TGAGATGGTA GATGGGGAGC 1861 CACCGTACTT CAGTGACTCC CCAGTGCAAG CCATGAAGAG GCTCCGGGAC AGCCCCCCAC 1921 CCAAGCTGAA AAACTCTCAC AAGGTCTCCC CAGTGCTGCG AGACTTCCTG GAGCGGATGC 1981 TGGTGCGGGA CCCCCAAGAG AGAGCCACAG CCCAGGAGCT CCTAGACCAC CCCTTCCTGC 2041 TGCAGACAGG GCTACCTGAG TGCCTGGTGC CCCTGATCCA GCTCTACCGA AAGCAGACCT 2101 CCACCTGCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2161 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2221 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACGGG TCGCCTACTC ATACCTGAAC 2281 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt