Construct: ORF TRCN0000474099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018905.2_s317c1
- Derived from:
- ccsbBroadEn_15059
- DNA Barcode:
- ACCCTCCAAGATTTCTGCGAAGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TNNI3K (51086)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474099
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51086 | TNNI3K | TNNI3 interacting kinase | NM_015978.3 | 99.5% | 70.2% | (many diffs) |
| 2 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001112808.2 | 87.1% | 60.7% | (many diffs) |
| 3 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001199327.1 | 76% | 67.2% | (many diffs) |
| 4 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | NM_177066.5 | 84.6% | 64.5% | (many diffs) |
| 5 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240154.1 | 73.6% | 73.7% | (many diffs) |
| 6 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240155.1 | 73.6% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1833
- ORF length:
- 1764
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaattat aaatctagac caacccaaac ttgtactgat gaatggaaga 121 aaaaagtcag tgaatcatat gttatcacaa tagaaagatt agaagatgac ctgcagatca 181 aggaaaaaga actgacagaa ctaaggaata tatttggctc tgatgaagcc ttcagtaaag 241 tcaatttaaa ttaccgcact gaaaatgggc tgtctctact tcatttatgt tgcatttgtg 301 gaggcaagaa atcacatatt cgaactctta tgttgaaagg gctccgccca tctcgactga 361 caagaaatgg atttacagcc ttgcatttag cagtttacaa ggataatgca gaattgatca 421 cttctctgct tcacagtgga gctgatatac agcaggttgg atacggtggc ctcactgccc 481 tccatattgc tacaatagct ggccacctag aggctgctga tgtgctgttg caacatggag 541 ctaatgtcaa tattcaagat gcagtttttt tcactccatt gcatattgca gcgtactatg 601 gacatgaaca ggtaactcgc cttcttttga aatttggtgc tgatgtaaat gtaagtggtg 661 aagttggaga tagacccctc cacctagcat ctgcaaaagg attcttgaat attgcaaaac 721 tcttgatgga agaacgcagc aaagcagatg tgaatgctca agataatgaa gaccatgtcc 781 cactccattt ctgttctcga tttggacacc atgatatagt taagtatctg ctgcaaagtg 841 atttggaagt tcaacctcat gttgttaata tctatggaga taccccctta cacctggcat 901 gctacaatgg caaatttgaa gttgccaagg aaatcatcca aatatcagga acagaaagtc 961 tgactaagga aaacatcttc agtgaaacag cttttcatag tgcttgtacc tatggcaaga 1021 gcattgacct agtcaaattt cttcttgatc agaatgtcat aaacatcaac caccaaggaa 1081 gggatgggca cactggatta cactctgctt gctaccacgg tcacattcgc ctggttcagt 1141 tcttactgga taatggagct gatatgaatc tagtggcttg tgatcccagc aggtctagtg 1201 gtgaaaaaga tgagcagaca tgtttgatgt gggcttatga aaaagggcat gatgccattg 1261 tcacactcct gaagcattat aagagaccac aagatgaatt gccctgtaat gaatattctc 1321 agcctggagg agatggctcc tatgtgtctg ttccatcacc cttggggaag attaaaagca 1381 tgacaaaaga gaaggcagat attctcctcc taagagctgg attgccttca catttccatc 1441 ttcagctctc agaaattgag ttccatgaga ttattggctc aggttctttt gggaaagtat 1501 ataaaggacg atgcagaaat aaaatagtgg ctataaaacg ttatcgagcc aatacctact 1561 gctccaagtc agatgtggat atgttttgcc gagaggtgtc cattctctgc cagctcaatc 1621 atccctgcgt aattcagttt gtgggtgctt gcttgaatga tcccagccag tttgccattg 1681 tcactcaata catatcaggg ggttctctgt tctccctcct tcatgagcag aagaggattc 1741 ttgatttgca gtctaaatta attattgcag tagatgttgc caaaggcatg gagtaccttc 1801 acaacctgac acagccaatt atacatcgtg actgaaacag tcacatattt ctttctctat 1861 gaggatgggc atgctgtgtg ggcagatttt gagaaatcaa gatttctaca gtctctggat 1921 gaagacaaca tgacaaaaca acctgggaac ctccgttgga tggctcntga ggtgttcacg 1981 cagtgcactc ggtacaccat caaagcagat gtcttcagct atgctctgtg tctgtgggaa 2041 attctcactg gcgaaattcc attcgctcat ctcaagccag cggctgcggc agcagacatg 2101 gcttaccacc acatcagacc tcccattggc tattccattc ccaagcccat atcatctctg 2161 ctGATACGAG GGTGGAACGC ATGTCCTGAA GGAAGACCCG AATTTTCTGA AGTTGTCATG 2221 AAGTTAGAAG AGTGTCTCTG CAACATTGAG CTGATGTCTC CTGCATCAAG TAACAGCAGT 2281 GGGTCTCTCT CACCTTCTTC TTCTTCTGAT TGCCTGGTGA ACCGGGGAGG ACCTGGCCGG 2341 AGTCATGTGG CAGCATTAAG AAGTCGTTTC GAATTGGAAT ATGCTCTAAA TGCAAGGTCC 2401 TATGCTGCTT TGTCCCAAAG TGCTGGACAA TATTCCTCTC AAGGTCTGTC TTTGGAGGAG 2461 ATGAAAAGAA GTCTTCAATA CACACCCATT GACAAATATG GCTATGTATC CGATCCCATG 2521 AGCTCAATGC ATTTTCATTC TTGCCGAAAT AGTAGCAGCT TTGAGGACAG CAGCTTGCCA 2581 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 2641 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 2701 ATATCTTGTG GAAAGGACGA ACCCTCCAAG ATTTCTGCGA AGACACGCGT TAAGTCgaca 2761 atcaacctct ggattacaaa atttgtgaaa gatt