Construct: ORF TRCN0000474112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017222.1_s317c1
- Derived from:
- ccsbBroadEn_09517
- DNA Barcode:
- CCTAAATTACCACCGGAGCAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP15 (128853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128853 | DUSP15 | dual specificity phosphatas... | NM_080611.4 | 99.8% | 99.5% | 589G>C |
| 2 | human | 128853 | DUSP15 | dual specificity phosphatas... | NM_001320478.1 | 97.5% | 97% | (many diffs) |
| 3 | human | 128853 | DUSP15 | dual specificity phosphatas... | XM_017027657.1 | 65.3% | 53.8% | (many diffs) |
| 4 | human | 128853 | DUSP15 | dual specificity phosphatas... | NM_001320479.1 | 63.7% | 52% | (many diffs) |
| 5 | human | 128853 | DUSP15 | dual specificity phosphatas... | XM_017027656.1 | 61.6% | 48.3% | (many diffs) |
| 6 | human | 128853 | DUSP15 | dual specificity phosphatas... | XM_017027654.1 | 57.4% | 45.2% | (many diffs) |
| 7 | human | 128853 | DUSP15 | dual specificity phosphatas... | XR_001754160.1 | 56.2% | (many diffs) | |
| 8 | human | 128853 | DUSP15 | dual specificity phosphatas... | XM_017027655.1 | 56% | 43.7% | (many diffs) |
| 9 | human | 128853 | DUSP15 | dual specificity phosphatas... | NM_001012644.2 | 56% | 55.7% | 0_1ins309;280G>C |
| 10 | human | 128853 | DUSP15 | dual specificity phosphatas... | NM_177991.3 | 56% | 55.7% | 0_1ins309;280G>C |
| 11 | human | 128853 | DUSP15 | dual specificity phosphatas... | XR_001754159.1 | 53.1% | (many diffs) | |
| 12 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | NM_001159376.1 | 86.9% | 89.3% | (many diffs) |
| 13 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XM_011239559.2 | 79.8% | 59.8% | (many diffs) |
| 14 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XM_011239560.2 | 77% | 49.4% | (many diffs) |
| 15 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XM_006499542.3 | 47.8% | 48.5% | (many diffs) |
| 16 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | NM_145744.2 | 39.1% | 35.2% | (many diffs) |
| 17 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XR_866278.2 | 20.7% | (many diffs) | |
| 18 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XR_866281.2 | 17.7% | (many diffs) | |
| 19 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XR_866279.2 | 17% | (many diffs) | |
| 20 | mouse | 252864 | Dusp15 | dual specificity phosphatas... | XR_374474.3 | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caatggcatg accaaggtac ttcctggact ctacctcgga aacttcattg 121 atgccaaaga cctggatcag ctgggccgaa ataagatcac acacatcatc tctatccatg 181 agtcacccca gcctctgctg caggatatca cctaccttcg catcccggtc gctgataccc 241 ctgaggtacc catcaaaaag cacttcaaag aatgtaTCAA CTTCATCCAC TGCTGCCGCC 301 TTAATGGGGG GAACTGCCTT GTGCACTGCT TTGCAGGCAT CTCTCGCAGC ACCACGATTG 361 TGACAGCGTA TGTGATGACT GTGACGGGGC TAGGCTGGCG GGACGTGCTT GAAGCCATCA 421 AGGCCACCAG GCCCATCGCC AACCCCAACC CAGGCTTTAG GCAGCAGCTT GAAGAGTTTG 481 GCTGGGCCAG TTCCCAGAAG CTTCGCCGGC AGCTGGAGGA GCGCTTCGGC GAGAGCCCCT 541 TCCGCGACGA GGAGGAGTTG CGCGCGCTGC TGCCGCTGTG CAAGCGCTGC CGGCAGGGCT 601 CCGCGACCTC GGCCTCCTCC GCCGGGCCGC ACTCAGCAGC CTCCGAGGGA ACCCTGCAGC 661 GCCTGGTGCC GCGCACGCCC CGGGAAGCCC ACCGGCCGCT GCCGCTGCTG GCGCGCGTCA 721 AGCAGACTTT CTCTTGCCTC CCCCGGTGTC TGTCCCGCAA GGGCGGCAAG TGCCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGACCTA AATTACCACC GGAGCAATCA ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt