Construct: ORF TRCN0000474135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002772.1_s317c1
- Derived from:
- ccsbBroadEn_12132
- DNA Barcode:
- GAACATGCGACACGCTATGTCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERC6 (55008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55008 | HERC6 | HECT and RLD domain contain... | XM_017008333.1 | 61.7% | 61.7% | 1_705del |
| 2 | human | 55008 | HERC6 | HECT and RLD domain contain... | NM_001165136.1 | 38.3% | 38.2% | 1_1818del;1820_1821delACinsTG;1824_1826delCCT |
| 3 | human | 55008 | HERC6 | HECT and RLD domain contain... | XM_011532053.3 | 37.9% | 37.7% | 1_1854del;1856_1857delACinsTG;1860_1862delCCT |
| 4 | human | 55008 | HERC6 | HECT and RLD domain contain... | NM_017912.3 | 37% | 37% | 1_1929del |
| 5 | human | 55008 | HERC6 | HECT and RLD domain contain... | XM_005263083.4 | 36.6% | 36.6% | 1_1965del |
| 6 | human | 55008 | HERC6 | HECT and RLD domain contain... | XR_001741267.2 | 28.2% | 1_2247del;3385_4024del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gatgtcagaa aagaaagcat acatgcttat gcatgaaaca attctgcaaa 121 aaaaggatga atttcctcca tcacccagat ttatacttag agtcagacga agtcgcctgg 181 ttaaagatgc tctgcgtcaa ttaagtcaag ctgaagctac tgacttctgc aaagtattag 241 tggttgaatt tattaatgaa atttgtcctg agtctggagg ggttagttca gagttcttcc 301 actgtatgtt tgaagagatg accaagccag aatatggaat gttcatgtat cctgaaatgg 361 gttcctgcat gtggtttcct gccaagccta aacctgagaa gaaaagatat ttcctctttg 421 gaatgctgtg tggactctcc ttattcaatt taaatgttgc taaccttcct ttcccactgg 481 ctctgtataa aaaacttctg gaccaaaagc catcattgga agatttaaaa gaactcagtc 541 ctcggttggg gaagagtttg caagaagttc tagatgatgc tgctgatgac attggagatg 601 cgctctgcat acgcttttct atacactggg accaaaatga tgttgactta attccaaatg 661 ggatctccat acctgtGGAC CAAACCAACA AGAGAGACTA TGTTTCTAAG TATATTGATT 721 ACATTTTCAA CGTCTCTGTA AAAGCAGTTT ATGAGGAATT TCAGAGAGGA TTTTATAGAG 781 TCTGTGAGAA GGAGATACTT AGACATTTCT ACCCTGAAGA ACTAATGACA GCAATCATTG 841 GAAATACTGA TTATGACTGG AAACAGTTTG AACAGAATTC AAAGTATGAG CAAGGATACC 901 AAAAATCACA TCCTACTATA CAGTTGTTTT GGAAGGCTTT CCACAAACTA ACCTTGGATG 961 AAAAGAAAAA ATTCCTCTTT TTCCTTACAG GACGTGATAG GCTGCATGCA AGAGGCATAC 1021 AGAAAATGGA AATAGTATTT CGCTGTCCTG AAACTTTCAG TGAAAGAGAT CACCCAACAT 1081 CAATAACTTG TCATAATATT CTCTCCCTCC CTAAGTATTC TACAATGGAA AGAATGGAGG 1141 AAGCACTTCA AGTAGCCATC AACAACAACA GAGGATTTGT CTCACCCATG CTCACACAGT 1201 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAGA ACATGCGACA CGCTATGTCG GCACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt