Construct: ORF TRCN0000474149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009928.1_s317c1
- Derived from:
- ccsbBroadEn_13513
- DNA Barcode:
- ATCTGTCATATTCATTACAGTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM171A2 (284069)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474149
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284069 | FAM171A2 | family with sequence simila... | NM_198475.3 | 9.8% | 2.9% | 1_184del;428_2478del |
| 2 | mouse | 217219 | Fam171a2 | family with sequence simila... | XM_006533049.3 | 8.7% | 2.6% | (many diffs) |
| 3 | mouse | 217219 | Fam171a2 | family with sequence simila... | NM_199200.2 | 8.6% | 2.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTG TTTGGGAACC GGACTCTGCT GGCAGCTGGC ACCACAGACT CAGAGGGTGT 121 GGCCACCCTG CCCCTCAGTT ATCGCTTGGG CACCTGGGTG CTGGTCACTG CTGCCCGCCC 181 TGGCTTCCTC ACCAACTCTG TGCCCTGGCG TGTTGACAAG CTGCCCTTGT ATGCGTCTGT 241 CAGCCTCTAC CTGCTCCCTG AGCGGCCGGC CACGCTCATC CTCTATGAGG ACCTGGTGCA 301 CATTCTCCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 361 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 421 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCTGT CATATTCATT ACAGTATAAC 481 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt