Construct: ORF TRCN0000474163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011241.1_s317c1
- Derived from:
- ccsbBroadEn_14115
- DNA Barcode:
- CCGATTCCTTGAGGCGATATTTAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B9D1 (27077)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321216.2 | 99.7% | 5% | 8delC |
| 2 | human | 27077 | B9D1 | B9 domain containing 1 | XM_005256610.2 | 92.5% | 3.1% | 8delC;402_403ins23;426_427insTGAGGACAAA |
| 3 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321219.2 | 88.1% | 1.3% | (many diffs) |
| 4 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321218.2 | 87.9% | 5% | (many diffs) |
| 5 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001330149.1 | 87.8% | 4.3% | (many diffs) |
| 6 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321217.2 | 78.6% | 7.5% | (many diffs) |
| 7 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321215.2 | 77.4% | 9% | (many diffs) |
| 8 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001321214.2 | 75.8% | 6.4% | (many diffs) |
| 9 | human | 27077 | B9D1 | B9 domain containing 1 | NM_015681.5 | 72.2% | 9.7% | (many diffs) |
| 10 | human | 27077 | B9D1 | B9 domain containing 1 | NM_001243473.2 | 52.4% | 47.4% | (many diffs) |
| 11 | mouse | 27078 | B9d1 | B9 protein domain 1 | NM_001330780.1 | 66.2% | 18.8% | (many diffs) |
| 12 | mouse | 27078 | B9d1 | B9 protein domain 1 | NM_013717.3 | 65.3% | 5.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacgcgagtc ctagcgtctt tctactcatg gtcaacgggc aggtggagag 121 cgccCAGTTT CCAGAGTATG ATGACCTCTA CTGCAAGTAC TGCTTTGTGT ACGGCCAGGA 181 CTGGGCCCCC ACAGCGGGTC TGGAGGAGGG GATCTCACAG ATCACATCCA AGAGCCAAGA 241 TGTGCGGCAA GCACTGGTGT GGAACTTCCC CATTGATGTC ACCTTTAAAA GCACCAACCC 301 CTACGGCTGG CCACAGATCG TGCTCAGCGT GTATGGACCA GATGTGTTCG GGAACGATGT 361 GGTTCGAGGC TATGGGGCCG TGCACGTGCC CTTCTCACCT GGCCGGCACA AAAGGACCAT 421 CCCCATGTTT GTCCCAGAAT CTACGTCTAA ACTGCAGAAG TTTACAAGTT TGTGCCTGGT 481 CGCCTCCTCA GATCTGCAAG CTGCTCCACC CACTGAGGAC AAATACCCAA CTTTCTTGTA 541 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 601 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 661 AAAGGACGAC CGATTCCTTG AGGCGATATT TACACGCGTT AAGTCgacaa tcaacctctg 721 gattacaaaa tttgtgaaag att