Construct: ORF TRCN0000474167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013067.1_s317c1
- Derived from:
- ccsbBroadEn_09358
- DNA Barcode:
- AGCCGCACTACAATACTCTCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK1IP1L (93487)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93487 | MAPK1IP1L | mitogen-activated protein k... | NM_144578.4 | 99.8% | 100% | 543A>G |
| 2 | human | 93487 | MAPK1IP1L | mitogen-activated protein k... | XM_017021789.2 | 97.7% | 97.1% | (many diffs) |
| 3 | human | 93487 | MAPK1IP1L | mitogen-activated protein k... | XM_011537362.2 | 94.8% | 94.9% | 1_39del;582A>G |
| 4 | mouse | 218975 | Mapk1ip1l | mitogen-activated protein k... | NM_178684.5 | 85.1% | 79.6% | (many diffs) |
| 5 | mouse | 218975 | Mapk1ip1l | mitogen-activated protein k... | XM_011245014.1 | 61.5% | 56.7% | (many diffs) |
| 6 | mouse | 218975 | Mapk1ip1l | mitogen-activated protein k... | XM_011245015.1 | 61.5% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgatgaattt tcgttggcag atgcactacc tgaacactcc cctgccaaaa 121 cctctgctgt gagcaataca aaacctggcc aacctcctca aggctggcca ggctccaacc 181 cttggaataa tccgagtgct ccatcttcag tgccatctgg actcccacca agtgcaacac 241 cctccactgt gccttttgga ccagcaccaa caggaatgta tccctccgtg cctcccaccg 301 gaccaccTCC AGGACCCCCA GCACCCTTTC CTCCTTCCGG ACCATCATGT CCCCCACCTG 361 GTGGTCCTTA TCCAGCCCCA ACTGTGCCGG GCCCTGGCCC CACAGGGCCA TATCCTACAC 421 CAAATATGCC CTTTCCAGAG CTACCCAGAC CATATGGTGC ACCCACAGAT CCAGCTGCAG 481 CTGGTCCTTT AGGTCCATGG GGATCCATGT CTTCTGGACC TTGGGCGCCA GGAATGGGAG 541 GGCAGTATCC TACCCCTAAT ATGCCATATC CATCTCCAGG CCCATATCCC GCTCCTCCTC 601 CTCCCCAGGC CCCTGGGGCA GCACCACCTG TTCCATGGGG CACCGTTCCA CCAGGAGCCT 661 GGGGACCACC AGCACCATAT CCTGCCCCTA CAGGATCGTA TCCCACACCA GGACTCTATC 721 CTACTCCCAG TAATCCTTTC CAAGTGCCTT CAGGACCTTC TGGTGCTCCA CCAATGCCTG 781 GTGGCCCCCA TTCTTACCAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCC GCACTACAAT 961 ACTCTCAATT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt