Construct: ORF TRCN0000474176
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001062.1_s317c1
- Derived from:
- ccsbBroadEn_10282
- DNA Barcode:
- GTTGTACAAACCCACTGCTGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEURL3 (93082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474176
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93082 | NEURL3 | neuralized E3 ubiquitin pro... | NM_001285485.2 | 52.2% | 6.2% | 1_61del;473_786del |
| 2 | human | 93082 | NEURL3 | neuralized E3 ubiquitin pro... | XM_011512178.2 | 52.2% | 6.2% | 1_61del;473_786del |
| 3 | human | 93082 | NEURL3 | neuralized E3 ubiquitin pro... | XM_011512179.2 | 52.2% | 6.2% | 1_61del;473_786del |
| 4 | human | 93082 | NEURL3 | neuralized E3 ubiquitin pro... | NR_104346.1 | 24.2% | 1_349del;761_1695del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 501
- ORF length:
- 411
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGAGGCC AAGGGCGCAC AGGTGCGTCT 121 GGACACGCGT GGCTGCATCG CGCACAGGCG CACCACGTTC CACGACGGCA TCGTGTTCAG 181 CCAGCGGCCG GTGCGCCTGG GCGAGCGTGT GGCGCTGCGA GTGCTGCGGG AGGAGAGCGG 241 CTGGTGCGGC GGCCTCCGCG TGGGCTTCAC GCGCCTGGAC CCCGCGTGCG TGTCCGTGCC 301 CAGCCTGCCG CCCTTCCTGT GCCCCGACCT GGAGGAGCAG AGCCCGACGT GGGCGGCCGT 361 GCTGCCTGAG GGCTGCGCGC TCACTGGGGA CTTGGTCCGC TTCTGGGTGG ACCGCCGCGG 421 CTGCCTCTTC GCCAAGGTCA ACGCCGGCTG CCGGCTCCTG CTGCGTGAGG GCGTGCCCGT 481 CGGCGCCCCG CTCTGGGCCG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTG TACAAACCCA 661 CTGCTGAATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt