Construct: ORF TRCN0000474177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012769.1_s317c1
- Derived from:
- ccsbBroadEn_12050
- DNA Barcode:
- GACTGTGTCCTTCTGTCAGATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APBB1IP (54518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54518 | APBB1IP | amyloid beta precursor prot... | XM_011519514.2 | 26.9% | 24.1% | (many diffs) |
| 2 | human | 54518 | APBB1IP | amyloid beta precursor prot... | NM_019043.4 | 24.9% | 22.4% | (many diffs) |
| 3 | human | 54518 | APBB1IP | amyloid beta precursor prot... | XM_006717451.2 | 24.9% | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgagtcaagt gaagacatag accaaatgtt cagcactttg ctgggagaga 121 tggatcttct gactcagagt ttaggagttg acactctccc tccTCCTGAC CCTAATCCAC 181 CCAGAGCTGA ATTTAACTAC AGTGTGGGGT TTAAAGATTT AAATGAGTCC TTAAATGCAC 241 TGGAAGACCA AGATTTAGAT GCTCTCATGG CAGATCTGGT AGCAGACATA AGTGAGGCTG 301 AGCAGAGGAC AATCCAGGCA CAGAAAGAGT CCTTGCAGAA TCAACATCAT TCAGCATCTC 361 TACAAGCATC AATTTTCAGT GGTGCAGCCT CTCTTGGTTA TGGAACAAAT GTTGCTGCCA 421 CTGGTATCAG CCAATATGAG GATGACTTAC CACCTCCACC AGCCGATCCT GTGTTAGACC 481 TTCCACTGCC ACCACCACCT CCTGAACCTC TCTCTCAGGT AAGTATGTGG GACCAGAGAT 541 GGCAGGACCA TCAACCTCTG CTACCTATCA CTGATGTTCC TTGCCCAACT TGCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAGAC TGTGTCCTTC TGTCAGATTG TACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t