Construct: ORF TRCN0000474183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008427.1_s317c1
- Derived from:
- ccsbBroadEn_11390
- DNA Barcode:
- CAAGGCTTTTGAGAGGACTGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABBR2 (9568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | NM_005458.8 | 93.7% | 93.7% | 1_174del;375T>G;2390G>A |
| 2 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_017015331.2 | 92.9% | 87.8% | (many diffs) |
| 3 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_005252316.5 | 77.3% | 77.2% | 0_1ins600;1616G>A |
| 4 | human | 9568 | GABBR2 | gamma-aminobutyric acid typ... | XM_017015332.2 | 77.3% | 77.2% | 0_1ins600;1616G>A |
| 5 | mouse | 242425 | Gabbr2 | gamma-aminobutyric acid (GA... | NM_001081141.1 | 83.9% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2715
- ORF length:
- 2649
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cctcatgccg ctcaccaagg aggtggccaa gggcagcatc gggcgcggtg 121 tgctccccgc cgtggaactg gccatcgagc agatccgcaa cgagtcactc ctgcgcccct 181 acttcctcga cctgcggctc tatgacacgg agtgcgacaa cgcaaaaggg ttgaaagcct 241 tctacgatgc aataaaatac gggccgaacc acttgatggt gtttggaggc gtctgtccat 301 ccgtcacatc catcattgca gagtccctcc aaggctggaa tctggtgcag ctttcttttg 361 ctgcaaccac gcctgttcta gccgataaga aaaaataccc ttatttcttt cggaccgtcc 421 catcagacaa tgcggtgaat ccagccattc tgaagttgct caagcactac cagtggaagc 481 gcgtgggcac gctgacgcaa gacgttcaga ggttctctga ggtgcggaat gacctgactg 541 gagttctgta tggcgaggac attgagattt cagacaccga gagcttctcc aacgatccct 601 gtaccagtgt caaaaagctg aaggggaatg atgtgcggat catccttggc cagtttgacc 661 agaatatggc agcaaaagtg ttctgttgtg catacgagga gaacatgtat ggtagtaaat 721 atcagtggat cattccgggc tggtacgagc cttcttggtg ggagcaggtg cacacggaag 781 ccaactcatc ccgctgcctc cggaagaatc tgcttgctgc catggagggc tacattggcg 841 tggatttcga gcccctgagc tccaagcaga tcaagaccat ctcaggaaag actccacagc 901 agtatgagag agagtacaac aacaagcggt caggcgtggg gcccagcaag ttccacgggt 961 acgcctacga tggcatctgg gtcatcgcca agacactgca gagggccatg gagacactgc 1021 atgccagcag ccggcaccag cggatccagg acttcaacta cacggaccac acgctgggca 1081 ggatcatcct caatgccatg aacgagacca acttcttcgg ggtcacgggt caagttgtat 1141 tccggaatgg ggagagaatg gggaccatta aatttactca atttcaagac agcagggagg 1201 tgaaggtggg agagtacaac gctgtggccg acacactgga gatcatcaat gacaccatca 1261 ggttccaagg atccgaacca ccaaaagaca agaccatcat cctggagcag ctgcggaaga 1321 tctccctacc tctctacagc atcctctctg ccctcaccat cctcgggatg atcatggcca 1381 gtgcttttct cttcttcaac atcaagaacc ggaatcagaa gctcataaag atgtcgagtc 1441 catacatgaa caaccttatc atccttggag ggatgctctc ctatgcttcc atatttctct 1501 ttggccttga tggatccttt gtctctgaaa agacctttga aacactttgc accgtcagga 1561 cctggattct caccgtgggc tacacgaccg cttttggggc catgtttgca aagacctgga 1621 gagtccacgc catcttcaaa aatgtgaaaa tgaagaagaa gatcatcaag gaccagaaac 1681 tgcttgtgat cgtggggggc atgctgctga tcgacctgtg tatcctgatc tgctggcagg 1741 ctgtggaccc cctgcgaagg acagtggaga agtacagcat ggagccggac ccagcaggac 1801 gggatatctc catccgccct ctcctggagc actgtgagaa cacccatatg accatctggc 1861 ttggcatcgt ctatgcctac aagggacttc tcatgttgtt cggttgtttc ttagcttggg 1921 agacccgcaa cgtcagcatc cccgcactca acgacagcaa gtacatcggg atgagtgtct 1981 acaacgtggg gatcatgtgc atcatcgggg ccgctgtctc cttcctgacc cgggaccagc 2041 ccaatgtgca gttctgcatc gtggctctgg tcatcatctt ctgcagcacc atcaccctct 2101 gcctggtatt cgtgccgaag ctcatcaccc tgagaacaaa cccagatgca gcaacgcaga 2161 acaggcgatt ccagttcact cagaatcaga agaaagaaga ttctaaaacg tccacctcgg 2221 tcaccagtgt gaaccaagcc agcacatccc gcctggaggg cctacagtca gaaaaccatc 2281 acctgcgaat gaagatcaca gagctggata aagacttgga agaggtcacc atgcagctgc 2341 aggacacacc agaaaagacc acctacatta aacagaacca ctaccaagag ctcaatgaca 2401 tcctcaacct gggaaacttc actgagagca cagatggagg aaaggccatt ttaaaaaatc 2461 acctcgatca aaatccccag ctacagtgga acacaacaga gccctctcga acatgcaaag 2521 atcctataga agatataaac tctccagaac acatccagcg tcggctgtcc ctccagctcc 2581 ccatcctcca ccacgcctac ctcccatcca tcggaggcgt ggacgccagc tgtgTCAGCC 2641 CCTGCGTCAG CCCCACCGCC AGCCCCCGCC ACAGACATGT GCCACCCTCC TTCCGAGTCA 2701 TGGTCTCGGG CCTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2761 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2821 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAAGGCTTTT GAGAGGACTG 2881 GAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt