Construct: ORF TRCN0000474209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000690.1_s317c1
- Derived from:
- ccsbBroadEn_05791
- DNA Barcode:
- GCATACACCGCATGCCTCAACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGT (183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474209
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 183 | AGT | angiotensinogen | NM_000029.4 | 99.7% | 99.5% | 803T>C;1003C>T;1116A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gaagcgagca ccccagtctg agatggctcc tgccggtgtg agcctgaggg 121 ccaccatcct ctgcctcctg gcctgggctg gcctggctgc aggtgaccgg gtgtacatac 181 accccttcca cctcgtcatc cacaatgaga gtacctgtga gcagctggca aaggccaatg 241 ccgggaagcc caaagacccc accttcatac ctgctccaat tcaggccaag acatcccctg 301 tggatgaaaa ggccctacag gaccagctgg tgctagtcgc tgcaaaactt gacaccgaag 361 acaagttgag ggccgcaatg gtcgggatgc tggccaactt cttgggcttc cgtatatatg 421 gcatgcacag tgagctatgg ggcgtggtcc atggggccac cgtcctctcc ccaacggctg 481 tctttggcac cctggcctct ctctatctgg gagccttgga ccacacagct gacaggctac 541 aggcaatcct gggtgttcct tggaaggaca agaactgcac ctcccggctg gatgcgcaca 601 aggtcctgtc tgccctgcag gctgtacagg gcctgctagt ggcccagggc agggctgata 661 gccaggccca gctgctgctg tccacggtgg tgggcgtgtt cacagcccca ggcctgcacc 721 tgaagcagcc gtttgtgcag ggcctggctc tctatacccc tgtggtcctc ccacgctctc 781 tggacttcac agaactggat gttgctgctg agaagattga caggttcatg caggctgtga 841 caggatggaa gactggctgc tccctgacgg gagccagtgt ggacagcacc ctggctttca 901 acacctacgt ccacttccaa gggaagatga agggcttctc cctgctggcc gagccccagg 961 agttctgggt ggacaacagc acctcagtgt ctgttcccat gctctctggc atgggcacct 1021 tccagcactg gagtgacatc caggacaact tctcggtgac tcaagtgtcc ttcactgaga 1081 gcgcctgcct gctgctgatc cagcctcact atgccTCTGA CCTGGACAAG GTGGAGGGTC 1141 TCACTTTCCA GCAAAACTCC CTCAACTGGA TGAAGAAACT GTCTCCCCGG ACCATCCACC 1201 TGACCATGCC CCAACTGGTG CTGCAAGGAT CTTATGACCT GCAGGACCTG CTCGCCCAGG 1261 CTGAGCTGCC CGCCATTCTG CACACCGAGC TGAACCTGCA AAAATTGAGC AATGACCGCA 1321 TCAGGGTGGG GGAGGTGCTG AACAGCATTT TTTTTGAGCT TGAAGCGGAT GAGAGAGAGC 1381 CCACAGAGTC TACCCAACAG CTTAACAAGC CTGAGGTCTT GGAGGTGACC CTGAACCGCC 1441 CATTCCTGTT TGCTGTGTAT GATCAAAGCG CCACTGCCCT GCACTTCCTG GGCCGCGTGG 1501 CCAACCCGCT GAGCACAGCA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAT ACACCGCATG 1681 CCTCAACTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt