Construct: ORF TRCN0000474223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011036.1_s317c1
- Derived from:
- ccsbBroadEn_12110
- DNA Barcode:
- CACTTGCGCGCGGGTTTTGTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM260 (54916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_001750384.2 | 70.3% | 1_123del;348G>T;1350_1741delinsA | |
| 2 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_017021379.2 | 65.2% | 65.1% | 225G>T;1227_1878delinsA |
| 3 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_011536851.2 | 64.2% | 64.1% | 225G>T;1227_1908delinsA |
| 4 | human | 54916 | TMEM260 | transmembrane protein 260 | NM_017799.4 | 57.7% | 57.7% | 225G>T;1227_2121delinsA |
| 5 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_011536852.1 | 37.2% | 34.6% | (many diffs) |
| 6 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_011536853.1 | 35.7% | 35.6% | 0_1ins468;759_1653delinsA |
| 7 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_245695.2 | 29.9% | 1_123del;348G>T;1351_4093del | |
| 8 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_001750387.2 | 28.8% | 1_123del;348G>T;1351_4248del | |
| 9 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_001750385.2 | 28.1% | 1_123del;348G>T;1351_4359del | |
| 10 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_001750382.2 | 27.7% | 1_123del;348G>T;1350_4420delinsA | |
| 11 | human | 54916 | TMEM260 | transmembrane protein 260 | XR_001750386.2 | 26.7% | 1_123del;348G>T;1351_4585del | |
| 12 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_017021380.1 | 20.2% | 20.1% | 0_1ins840;387_1068delinsA |
| 13 | human | 54916 | TMEM260 | transmembrane protein 260 | XM_006720176.1 | 18.1% | 18.1% | 0_1ins840;387_1281delinsA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1296
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtccccat ggcgacggca ggggccaggc ccaggggcgg gcagtccgag 121 tggggctgcg gcgctccggg ggcatccgcg gcggcgtggc ggtgttcgcc gccgtggccg 181 cagtgttcac cttcaccctg cccccttcgg taccgggggg agactccggg gaactgatca 241 cagccgcaca tgagcttgga gttgcccatc ctcctggcta tcctttgttc actctggtgg 301 ctaaactggc aattacactg tttccttttg gttcaattgc ctaccgcgtc aatcttctct 361 gtggcttatt tggagcagta gctgcatcat tacttttttt caccgttttc aggctttctg 421 gctcatctgc tggaggaatc cttgctgcgg gggtgttttc attttctcgt ctaacatggc 481 agtggtccat tgcagcagag gtttttagct taaacaatct ctttgtgggg ctgcttatgg 541 ctcttactgt acattttgag gaagcagcaa ctgctaagga gagatcaaag gtagctaaaa 601 ttggtgcttt ctgctgtggc cttagtttat gtaaccagca cacaataata ctctatgttt 661 tgtgcataat accttggatt ctctttcaac ttttaaaaaa gaaggaactc tccctgggct 721 ctttgttgaa gttgagcctg tacttctctg ctggtttgct gccctatgtc caCCTTCCCA 781 TCTCATCTTA CCTTAATCAC GCCCGGTGGA CCTGGGGAGA CCAGACAACA CTGCAAGGAT 841 TTTTGACACA TTTTCTCAGG GAAGAATATG GAACCTTCAG CCTGGCCAAA TCTGAAATAG 901 GATCCAGTAT GTCTGAAATA CTGCTTTCTC AAGTAACAAA TATGAGGACC GAACTCTCAT 961 TCAACATCCA AGCCCTTGCA GTTTGTGCAA ATATATGTTT AGCAACAAAG GACAGACAGA 1021 ATCCATCATT AGTATGGCTT TTTACTGGAA TGTTTTGCAT TTATTCATTG TTCTTTGCTT 1081 GGAGAGCAAA TTTAGATATT TCAAAACCAC TTTTCATGGG TGTGGTGGAA CGATTCTGGA 1141 TGCAGAGCAA TGCAGTAGTG GCCGTCCTCG CTGGCATTGG TTTGGCTGCA GTTGTGTCTG 1201 AGACTAACCG AGTGCTGAAT AGCAATGGGC TTCAGTGTCT GGAATGGCTT TCTGCAACTC 1261 TTTTTGTAGT TTACCAAATA TATTCTAATT ACAGATTGCC AACTTTCTTG TACAAAGTGG 1321 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1381 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1441 ACACTTGCGC GCGGGTTTTG TCGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1501 aatttgtgaa agatt