Construct: ORF TRCN0000474236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003478.1_s317c1
- Derived from:
- ccsbBroadEn_00929
- DNA Barcode:
- AGACTTTATTTAAGTCTTCTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LBR (3930)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3930 | LBR | lamin B receptor | NM_002296.4 | 100% | 100% | |
| 2 | human | 3930 | LBR | lamin B receptor | NM_194442.2 | 100% | 100% | |
| 3 | human | 3930 | LBR | lamin B receptor | XM_011544185.3 | 100% | 100% | |
| 4 | human | 3930 | LBR | lamin B receptor | XM_005273125.3 | 93.1% | 93.1% | 1187_1188ins126 |
| 5 | human | 3930 | LBR | lamin B receptor | XR_001737168.2 | 46.1% | 1_127del;1210_1211ins104;1869_3672del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1911
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aagtaggaaa tttgccgatg gtgaagtggt aagaggtcga tggcctggga 121 gttcacttta ttatgaagta gaaattctga gccacgacag cacctcccag ctttacactg 181 tgaagtataa agatggaaca gagcttgaat tgaaagagaa tgatattaag cctttaactt 241 cctttaggca aaggaaaggt ggctcaactt ccagttcccc ttccagacgc cgagggagtc 301 gatcaaggtc acgctcccga tcccctggtc gaccacctaa aagtgcccgc cgatctgctt 361 ctgcttccca ccaggccgac attaaggaag caaggaggga agtggaagtt aaattgactc 421 cgctgattct gaagccattt ggaaatagca tcagcagata taatggggag cctgagcata 481 ttgagagaaa tgacgcacct cataaaaata cacaggaaaa attcagtttg tcacaagaaa 541 gcagttacat agcaacacag tatagccttc gtccaagaag agaagaagtc aaattaaaag 601 aaatagattc taaggaagaa aaatacgttg caaaagaact ggcagtgaga acctttgaag 661 tgacccccat ccgggcaaag gacttggagt ttggaggagt acctggtgtg tttctcatca 721 tgtttggcct gcctgtgttc ctcttcctgt tgctgttgat gtgtaaacag aaagatccca 781 gtcttctgaa tttccctcct cctttgccag ctttgtatga gttatgggaa accagagtat 841 ttggggtcta cctcctgtgg tttttgattc aagtcctgtt ctacctactg ccaattggaa 901 aggttgtaga aggaacgcct cttattgatg gaagaagact caagtataga ttaaatggat 961 tctatgcttt tatcctgaca tctgcagtca tcggaacatc tctcttccag ggcgtagagt 1021 ttcattacgt gtacagtcat tttcttcagt ttgcacttgc ggccactgtt ttttgtgtgg 1081 tcttgagtgt gtatctctac atgcgctctt tgaaagcgcc ccggaatgac ctgtcgcctg 1141 ccagctctgg aaatgctgtc tatgatttct tcattggccg tgaattaaac cctcgaattg 1201 gtacttttga tctcaaatac ttttgtgaat tgcgccccgg attgattgga tgggtggtta 1261 ttaacttggt gatgcttttg gctgaaatga aaatacagga ccgcgctgtt ccatccttgg 1321 ccatgatttt agttaatagt ttccagcttc tctatgtggt ggatgctctc tggaatgagg 1381 aAGCGTTGTT GACGACCATG GACATCATCC ACGATGGATT TGGATTCATG CTGGCTTTTG 1441 GAGACTTGGT GTGGGTTCCC TTTATTTACA GCTTCCAAGC CTTTTATTTA GTCAGTCATC 1501 CAAATGAAGT GTCTTGGCCA ATGGCTTCTC TAATTATTGT TCTGAAACTT TGTGGTTATG 1561 TAATCTTCCG AGGTGCAAAT TCTCAGAAAA ATGCATTCCG GAAAAATCCC AGTGATCCAA 1621 AGCTTGCACA TTTAAAAACC ATTCATACTT CAACGGGAAA AAATCTTCTA GTTTCTGGAT 1681 GGTGGGGCTT TGTTCGCCAC CCCAATTACT TGGGTGATCT CATCATGGCC TTGGCGTGGT 1741 CCCTCCCATG TGGTTTTAAC CACATTCTGC CTTATTTCTA CATAATTTAT TTCACCATGT 1801 TGCTTGTCCA CCGAGAAGCT CGTGACGAGT ACCACTGTAA GAAGAAATAC GGCGTGGCTT 1861 GGGAAAAGTA CTGTCAGCGT GTGCCCTACC GTATATTTCC ATACATCTAC TACCCAACTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGAAGAC TTTATTTAAG TCTTCTAAAG ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt