Construct: ORF TRCN0000474239

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004022.1_s317c1
Derived from:
ccsbBroadEn_05783
DNA Barcode:
CCCGTTGACTATAGAAGCCAATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADORA3 (140)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474239
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 140 ADORA3 adenosine A3 receptor NM_000677.4 99.7% 100% 882T>C;897T>C
2 human 140 ADORA3 adenosine A3 receptor NM_001302679.2 54.1% 54.4% 0_1ins435;447T>C;462T>C
3 human 140 ADORA3 adenosine A3 receptor NM_001302678.2 38.2% 36.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1020
ORF length:
954
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc caacaacagc actgctctgt cattggccaa tgttacctac atcaccatgg
121aaattttcat tggactctgc gccatagtgg gcaacgtgct ggtcatctgc gtggtcaagc
181tgaaccccag cctgcagacc accaccttct atttcattgt ctctctagcc ctggctgaca
241ttgctgttgg ggtgctggtc atgcctttgg ccattgttgt cagcctgggc atcacaatcc
301acttctacag ctgccttttt atgacttgcc tactgcttat ctttacccac gcctccatca
361tgtccttgct ggccatcgct gtggaccgat acttgcgggt caagcttacc gtcagataca
421agagggtcac cactcacaga agaatatggc tggccctggg cctttgctgg ctggtgtcat
481tcctggtggg attgaccccc atgtttggct ggaacatgaa actgacctca gagtaccaca
541gaaatgtcac cttCCTTTCA TGCCAATTTG TTTCCGTCAT GAGAATGGAC TACATGGTAT
601ACTTCAGCTT CCTCACCTGG ATTTTCATCC CCCTGGTTGT CATGTGCGCC ATCTATCTTG
661ACATCTTTTA CATCATTCGG AACAAACTCA GTCTGAACTT ATCTAACTCC AAAGAGACAG
721GTGCATTTTA TGGACGGGAG TTCAAGACGG CTAAGTCCTT GTTTCTGGTT CTTTTCTTGT
781TTGCTCTGTC ATGGCTGCCT TTATCTATCA TCAACTGCAT CATCTACTTT AATGGTGAGG
841TACCACAGCT TGTGCTGTAC ATGGGCATCC TGCTGTCCCA TGCCAACTCC ATGATGAACC
901CTATCGTCTA TGCCTATAAA ATAAAGAAGT TCAAGGAAAC CTACCTCTTG ATCCTCAAAG
961CCTGTGTGGT CTGCCATCCC TCTGATTCTT TGGACACAAG CATTGAGAAG AATTCTGAGT
1021ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1081GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1141TTTATATATC TTGTGGAAAG GACGACCCGT TGACTATAGA AGCCAATAAA CGCGTTAAGT
1201Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)