Construct: ORF TRCN0000474239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004022.1_s317c1
- Derived from:
- ccsbBroadEn_05783
- DNA Barcode:
- CCCGTTGACTATAGAAGCCAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADORA3 (140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140 | ADORA3 | adenosine A3 receptor | NM_000677.4 | 99.7% | 100% | 882T>C;897T>C |
| 2 | human | 140 | ADORA3 | adenosine A3 receptor | NM_001302679.2 | 54.1% | 54.4% | 0_1ins435;447T>C;462T>C |
| 3 | human | 140 | ADORA3 | adenosine A3 receptor | NM_001302678.2 | 38.2% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caacaacagc actgctctgt cattggccaa tgttacctac atcaccatgg 121 aaattttcat tggactctgc gccatagtgg gcaacgtgct ggtcatctgc gtggtcaagc 181 tgaaccccag cctgcagacc accaccttct atttcattgt ctctctagcc ctggctgaca 241 ttgctgttgg ggtgctggtc atgcctttgg ccattgttgt cagcctgggc atcacaatcc 301 acttctacag ctgccttttt atgacttgcc tactgcttat ctttacccac gcctccatca 361 tgtccttgct ggccatcgct gtggaccgat acttgcgggt caagcttacc gtcagataca 421 agagggtcac cactcacaga agaatatggc tggccctggg cctttgctgg ctggtgtcat 481 tcctggtggg attgaccccc atgtttggct ggaacatgaa actgacctca gagtaccaca 541 gaaatgtcac cttCCTTTCA TGCCAATTTG TTTCCGTCAT GAGAATGGAC TACATGGTAT 601 ACTTCAGCTT CCTCACCTGG ATTTTCATCC CCCTGGTTGT CATGTGCGCC ATCTATCTTG 661 ACATCTTTTA CATCATTCGG AACAAACTCA GTCTGAACTT ATCTAACTCC AAAGAGACAG 721 GTGCATTTTA TGGACGGGAG TTCAAGACGG CTAAGTCCTT GTTTCTGGTT CTTTTCTTGT 781 TTGCTCTGTC ATGGCTGCCT TTATCTATCA TCAACTGCAT CATCTACTTT AATGGTGAGG 841 TACCACAGCT TGTGCTGTAC ATGGGCATCC TGCTGTCCCA TGCCAACTCC ATGATGAACC 901 CTATCGTCTA TGCCTATAAA ATAAAGAAGT TCAAGGAAAC CTACCTCTTG ATCCTCAAAG 961 CCTGTGTGGT CTGCCATCCC TCTGATTCTT TGGACACAAG CATTGAGAAG AATTCTGAGT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGACCCGT TGACTATAGA AGCCAATAAA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt