Construct: ORF TRCN0000474247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006779.1_s317c1
- Derived from:
- ccsbBroadEn_13648
- DNA Barcode:
- TGACACCCGTCGTTGATCTCTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIPK1A (388650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388650 | DIPK1A | divergent protein kinase do... | NM_001252273.1 | 100% | 100% | |
| 2 | human | 388650 | DIPK1A | divergent protein kinase do... | NM_001252270.1 | 38.8% | 28.7% | (many diffs) |
| 3 | human | 388650 | DIPK1A | divergent protein kinase do... | NM_001006605.5 | 37.6% | 37.3% | (many diffs) |
| 4 | human | 388650 | DIPK1A | divergent protein kinase do... | NM_001252271.1 | 31.8% | 31.5% | (many diffs) |
| 5 | human | 388650 | DIPK1A | divergent protein kinase do... | NM_001252269.1 | 27.1% | 26.8% | (many diffs) |
| 6 | mouse | 67266 | Fam69a | family with sequence simila... | NM_026062.4 | 34.1% | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaggagtctc tgtccggggg cctggctaag gaaaccctat tacctccagg 121 ctcgcttcTC ATATGTGCGG ATGAAATATC TTTTCTTTTC CTGGTTAGTG GTTTTTGTTG 181 GAAGCTGGAT TATATATGTG CAGTATTCTA CCTATACAGA ATTATGCAGA GGAAAGGACT 241 GTAAGAAAAT AATATGTGAC AAGTACAAGA CTGGAGTTAT TGATGGGCCT GCATGTAACA 301 GCCTTTGTGT TACAGAAACT CTTTACTTTG GAAAATGTTT ATCCACCAAG CCCAACAATC 361 AGATGTATTT AGGGATTTGG GATAATCTAC CAGGTGTTGT GAAATGTCAA ATGGAACAAG 421 CGCTTCATCT TGATTTTGGA ACTGAATTGG AACCAAGAAA AGAAATAGTG CTATTTGATA 481 AGCCAACTAG AGGAACTACT GTACAAAAAT TTAAAGAAAT GGTCTATAGT CTCTTTAAGG 541 ACCAGCACCG GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA CACCCGTCGT TGATCTCTCT 721 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t