Construct: ORF TRCN0000474273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010971.1_s317c1
- Derived from:
- ccsbBroadEn_07128
- DNA Barcode:
- GTTTAGTATAACTCTTATATGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VHL (7428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7428 | VHL | von Hippel-Lindau tumor sup... | NM_198156.3 | 99.8% | 99.4% | 328C>T |
| 2 | human | 7428 | VHL | von Hippel-Lindau tumor sup... | NM_000551.3 | 80.5% | 80.2% | 328C>T;339_461del |
| 3 | human | 7428 | VHL | von Hippel-Lindau tumor sup... | NM_001354723.2 | 74.8% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGCC CCGGAGGGCG GAGAACTGGG ACGAGGCCGA GGTAGGCGCG GAGGAGGCAG 121 GCGTCGAAGA GTACGGCCCT GAAGAAGACG GCGGGGAGGA GTCGGGCGCC GAGGAGTCCG 181 GCCCGGAAGA GTCCGGCCCG GAGGAACTGG GCGCCGAGGA GGAGATGGAG GCCGGGCGGC 241 CGCGGCCCGT GCTGCGCTCG GTGAACTCGC GCGAGCCCTC CCAGGTCATC TTCTGCAATC 301 GCAGTCCGCG CGTCGTGCTG CCCGTATGGC TCAACTTCGA CGGCGAGCCG CAGCCCTACC 361 CAACGCTGCC GCCTGGCACG GGCCGCCGCA TCTACAGCTA CCGAGTGTAT ACTCTGAAAG 421 AGCGATGCCT CCAGGTTGTC CGGAGCCTAG TCAAGCCTGA GAATTACAGG AGACTGGACA 481 TCGTCAGGTC GCTCTACGAA GATCTGGAAG ACCACCCAAA TGTGCAGAAA GACCTGGAGC 541 GGCTGACACA GGAGCGCATT GCACATCAAC GGATGGGAGA TTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAGTT TAGTATAACT CTTATATGGT TACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t