Construct: ORF TRCN0000474277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008881.1_s317c1
- Derived from:
- ccsbBroadEn_06719
- DNA Barcode:
- CAAGCCTGTGAGCAACCTTCGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGM3 (5238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_015599.3 | 99.8% | 99.8% | 1281A>G;1396G>A |
| 2 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_001199919.2 | 95.5% | 95.4% | 1281A>G;1396G>A;1625_1698delinsTC |
| 3 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_001199917.2 | 94.9% | 94.9% | 1_84del;1365A>G;1480G>A |
| 4 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_001367287.1 | 94.9% | 94.9% | 1_84del;1365A>G;1480G>A |
| 5 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_001367286.1 | 92.3% | 92.2% | 1239_1240ins123;1273G>A |
| 6 | human | 5238 | PGM3 | phosphoglucomutase 3 | XM_017010935.1 | 81.2% | 81% | (many diffs) |
| 7 | human | 5238 | PGM3 | phosphoglucomutase 3 | NM_001199918.2 | 80.7% | 79.1% | (many diffs) |
| 8 | human | 5238 | PGM3 | phosphoglucomutase 3 | XR_001743468.2 | 77.4% | (many diffs) | |
| 9 | human | 5238 | PGM3 | phosphoglucomutase 3 | XM_017010937.1 | 74% | 73.8% | (many diffs) |
| 10 | human | 5238 | PGM3 | phosphoglucomutase 3 | NR_159812.2 | 51.1% | (many diffs) | |
| 11 | human | 5238 | PGM3 | phosphoglucomutase 3 | XR_942480.2 | 47.8% | (many diffs) | |
| 12 | mouse | 109785 | Pgm3 | phosphoglucomutase 3 | NM_028352.4 | 83.9% | 87.4% | (many diffs) |
| 13 | mouse | 109785 | Pgm3 | phosphoglucomutase 3 | NM_001163746.1 | 77.3% | 80.4% | (many diffs) |
| 14 | mouse | 109785 | Pgm3 | phosphoglucomutase 3 | XM_017313098.1 | 54.5% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1692
- ORF length:
- 1626
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttaggtgct attacaaaat actcagcatt acacgccaag cccaatggac 121 tgatccttca atacgggact gctggatttc gaacgaaggc agaacatctt gatcatgtca 181 tgtttcgcat gggattatta gctgtcctga ggtcaaaaca gacaaaatcc actataggag 241 tcatggtaac agcgtcccac aatcctgagg aagacaatgg tgtaaaattg gttgatcctt 301 tgggtgaaat gttggcacca tcctgggagg aacatgccac ctgtttagca aatgctgagg 361 aacaagatat gcagagagtg cttattgaca tcagcgagaa agaagctgtg aatctgcaac 421 aagatgcctt tgtagttatt ggtagagata ccaggcccag cagtgagaaa ctttcacaat 481 ctgtaataga tggtgtgact gttctaggag gtcaattcca tgattatggc ttgttaacaa 541 caccccagct gcactacatg gtgtattgtc gaaacacggg tggccgatat ggaaaggcaa 601 ctatagaagg ttactaccag aaactctcta aggcttttgt ggaactcacc aaacaggctt 661 cttgcagtgg agatgaatac agatcactta aggttgactg tgcaaatggc ataggggccc 721 tgaagctaag ggaaatggaa cactacttct cacagggcct gtcagttcag ctgtttaatg 781 atgggtccaa gggcaaactc aatcatttat gtggagctga ctttgtgaaa agtcatcaga 841 aacctccaca gggaatggaa attaagtcca atgaaagatg ctgttctttt gatggagatg 901 cagacagaat tgtttattac taccatgatg cagatggcca ctttcatctc atagatggag 961 acaagatagc aacgttaatt agcagtttcc ttaaagagct cctggtggag attggagaaa 1021 gtttgaatat tggtgttgta caaactgcat atgcaaatgg aagttcaaca cggtatcttg 1081 aagaagttat gaaggtacct gtctattgca ctaagactgg TGTAAAACAT TTGCACCACA 1141 AGGCTCAAGA GTTTGACATT GGAGTTTATT TTGAAGCAAA TGGGCATGGC ACTGCACTGT 1201 TTAGTACAGC TGTTGAAATG AAGATAAAAC AATCAGCAGA ACAACTGGAA GATAAGAAAA 1261 GAAAAGCTGC TAAGATGCTT GAAAACATTA TTGACTTGTT TAACCAGGCA GCTGGTGATG 1321 CTATTTCTGA CATGCTGGTG ATTGAGGCAA TCTTGGCTCT GAAGGGCTTG ACTGTACAAC 1381 AGTGGGATGC TCTCTATACA GATCTTCCAA ACAGACAACT TAAAGTTCAG GTTGCAGACA 1441 GGAGAGTTAT TAGCACTACC AATGCTGAAA GACAAGCAGT TACACCCCCA GGATTACAGG 1501 AGGCAATCAA TGACCTGGTG AAGAAGTACA AGCTTTCTCG AGCTTTTGTC CGGCCCTCTG 1561 GTACAGAAGA TGTCGTCCGA GTATATGCAG AAGCAGACTC ACAAGAAAGT GCAGATCACC 1621 TTGCACATGA AGTGAGCTTG GCAGTATTTC AGCTGGCTGG AGGAATTGGA GAAAGGCCCC 1681 AACCAGGTTT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1741 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1801 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAA GCCTGTGAGC AACCTTCGGA 1861 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t